The laboratory is equipped for molecular biology; protein purification (AKTAPrime plus) and expression in bacteria, insect and mammalian cells; immunblotting and gel documentation (Syngene PXi); patch-clamp recording with fluorescence; bilayer and patch-clamp recording (PortaPatch); fluorescence polarization measurements (PHERAStar); high-throughput fluorescence measurements in 96-well plates (FlexStation 3, Corbett robot); use of radiochemicals (including Brandel receptor-binding harvesters).
Our microscopy facilities (Olympus) include single-cell fluorescence imaging, time-lapse recording with environmental cabinet, TIRF microscope with environmental cabinet and flash photolysis equipment, and a spinning-disc confocal microscope equipped to allow TIRF, PALM and flash photolysis of caged compounds. The microscopes are supported by off-line workstations.
Antibodies and plasmids
Please address requests for reagents to Professor Taylor (firstname.lastname@example.org). When submitting a request, please include the published reference, code/name of the reagent, and its intended use.
Images and movies
This area is presently under development