skip to primary navigationskip to content
 

Visualizing and controlling the cell cycle machinery in single cells

When Dec 04, 2017
from 03:00 PM to 04:00 PM
Where Pharmacology Lecture Theatre
Contact Name
Contact Phone +44 (0) 1223 3 33964
Attendees All welcome
Add event to calendar vCal
iCal

Cell cycle kinetics are crucial to cell fate decisions. Although live imaging has provided extensive insights into this relationship at the single-cell level, the limited number of fluorescent markers that can be used in a single experiment has hindered efforts to link the dynamics of individual proteins responsible for decision making directly to cell cycle progression. I will present an all-in-one cell cycle reporter that allows simultaneous analysis of cell cycle progression, including the transition into quiescence, and the dynamics of individual fate determinants. Focusing on cyclin D1 as a major cell cycle regulator, we present evidence that a crucial cell cycle function of cyclin D1 is to prevent the transition into quiescence and identify a unique cyclin D1 protein kinetics that are indicative of very short G1 phases. In addition, to allow manipulating the levels of endogenous proteins at will, we introduce a novel approach to render already existing GFP (trap) lines amenable to auxin-dependent degradation by simple ectopic expression of a degradation cassette using human cells and zebrafish as a proofs of principle.

More information about this event…

RSS Feed Latest news

Awareness Book Club (ABC)

Jul 27, 2020

We are pleased to officially launch the Awareness Book Club (ABC) today with the first book...

Rosetrees Trust funded project

Jul 21, 2020

Dr Cathy Wilson and Dr Paul Miller are awarded Rosetrees-Cambridge Innovation & Translation 2020 Awards to pioneer approaches in cardiac regeneration and CNS antibody delivery, respectively...

Dr Sampurna Chakrabarti

Jul 20, 2020

Dr Sampurna Chakrabarti awarded Alexander von Humboldt Fellowship ...

CONGRATULATIONS!

Jul 07, 2020

Ana Rossi and Janet Kumita

Transformation of naked mole-rat cells

Jul 01, 2020

Dr. Walid Khaled & Dr. Ewan Smith had a paper accepted in Nature that has been featured by the University and the Guardian...

View all news