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Department of Pharmacology

 
Author(s): 
Jeggle, P, Smith, ESJ, Stewart, AP, Haerteis, S, Korbmacher, C, Edwardson, JM
Abstract: 

ASIC and ENaC are co-expressed in various cell types, and there is evidence for a close association between them. Here, we used atomic force microscopy (AFM) to determine whether ASIC1a and ENaC subunits are able to form cross-clade hybrid ion channels. ASIC1a and ENaC could be co-isolated from detergent extracts of tsA 201 cells co-expressing the two subunits. Isolated proteins were incubated with antibodies against ENaC and Fab fragments against ASIC1a. AFM imaging revealed proteins that were decorated by both an antibody and a Fab fragment with an angle of ∼120° between them, indicating the formation of ASIC1a/ENaC heterotrimers.

Publication ID: 
681634
Published date: 
14 August 2015
Publication source: 
manual
Publication type: 
Journal articles
Journal name: 
Biochemical and Biophysical Research Communications
Publication volume: 
464
Publisher: 
Elsevier
Parent title: 
Edition: 
Publication number: