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Department of Pharmacology

 
Author(s): 
Arrand, JE, Willis, AE, Goldsmith, I, Lindahl, T
Abstract: 

Mammalian cells contain the DNA ligases I and II. These enzymes show different molecular weights and heat labilities, and antibodies against ligase I do not inhibit ligase II. Here, the nonidentical substrate specificities of the enzymes are described. Under standard reaction conditions DNA ligase I, but not ligase II, catalyzes blunt-end joining of DNA, while ligase II is the only activity that joins oligo(dT) molecules hydrogen-bonded to poly(rA). These differences facilitate the distinction between the two enzymes and should permit further analysis of their functions.

Publication ID: 
1377148
Published date: 
15 July 1986
Publication source: 
pubmed
Publication type: 
Journal articles
Journal name: 
J Biol Chem
Publication volume: 
261
Publisher: 
Parent title: 
Edition: 
Publication number: