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Department of Pharmacology

 
Author(s): 
Prole, DL, López-Sanjurjo, CI, Tovey, SC, Taylor, CW
Abstract: 

The endoplasmic reticulum (ER) is both the major source of intracellular Ca(2+) for cell signaling and the organelle that forms the most extensive contacts with the plasma membrane and other organelles. Lysosomes fulfill important roles in degrading cellular materials and in cholesterol handling, but they also contribute to Ca(2+) signaling by both releasing and sequestering Ca(2+). Interactions between ER and other Ca(2+)-transporting membranes, notably mitochondria and the plasma membrane, often occur at sites where the two membranes are closely apposed, allowing local Ca(2+) signaling between them. These interactions are often facilitated by scaffold proteins. Recent evidence suggests similar local interactions between ER and lysosomes. We describe simple fluorescence-based methods that allow the interplay between Ca(2+) signals, the ER, and lysosomes to be examined.

Publication ID: 
667354
Published date: 
December 2015
Publication source: 
pubmed
Publication type: 
Journal articles
Journal name: 
Methods Cell Biol
Publication volume: 
126
Publisher: 
Parent title: 
Edition: 
Publication number: