A novel approach to an amperometrlc enzyme electrode for the analysis of glucose is described. The technique entails the electrochemical codeposltfon of the redox enzyme, glucose oxidase, In the conducting organic polymer, polypyrrole. Reagentless glucose electrodes have been generated by the synthesis of N-subStltuted pyrrole monomers containing redox-active side chains designed to accept electrons from the reduced form of the enzyme. Ferrocene-pyrrole conjugates were found to be efficient oxidants of reduced glucose oxidase, which on anodic polymerization form redox-active films. Preliminary results suggest that the enzyme may be entrapped in ferrocene-containing polypyrrole films to construct a reagentless glucose electrode. © 1988, American Chemical Society. All rights reserved.
