Preparative affinity precipitation of L-lactate dehydrogenase

The methoxylated p-sulphonate isomer of the triazine dye C.I. Reactive Blue 2, selectively precipitates L-lactate dehydrogenase from crude rabbit muscle extracts. At mildly alkaline pH values and a 7-fold molar excess of the dye analogue to enzyme subunits, 106 mg of homogeneous lactate dehydrogenase essentially free of soluble ligands and the principal contaminating enzyme activities, may be obtained in 60% overall yield from 100 g tissue in less than 3 h. © 1989.