A smartphone application algorithm with inter-phone repeatability was developed for both Android and iOS operating systems. The app transformed the smartphone into a reader to quantify commercial colorimetric urine tests with high accuracy and reproducibility in measuring pH, protein, and glucose. The results showed linear responses in the ranges of 5.0-9.0, 0-100 mg/dL and 0-300 mg/dL, respectively. © 2014 Elsevier B.V.