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Department of Pharmacology

 
Author(s): 
Monette, M, Opella, SJ, Greenwood, J, Willis, AE, Perham, RN
Abstract: 

The NANP repeating sequence of the circumsporozoite protein of Plasmodium falciparum was displayed on the surface of fd filamentous bacteriophage as a 12-residue insert (NANP)(3) in the N-terminal region of the major coat protein (pVIII). The structure of the epitope determined by multidimensional solution NMR spectroscopy of the modified pVIII protein in lipid micelles was shown to be a twofold repeat of an extended and non-hydrogen-bonded loop based on the sequence NPNA, demonstrating that the repeating sequence is NPNA, not NANP. Further, high resolution solid-state NMR spectra of intact hybrid virions containing the modified pVIII proteins demonstrate that the peptides displayed on the surface of the virion adopt a single, stable conformation; this is consistent with their pronounced immunogenicity as well as their ability to mimic the antigenicity of their native parent proteins.

Publication ID: 
971815
Published date: 
June 2001
Publication source: 
pubmed
Publication type: 
Journal articles
Journal name: 
Protein Sci
Publication volume: 
10
Publisher: 
Parent title: 
Edition: 
Publication number: