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  • An Improved Targeted cAMP Sensor to Study the Regulation of Adenylyl Cyclase 8 by Ca2+ Entry through Voltage-Gated Channels. Everett KL, Cooper DMF.  PLoS ONE 8(9): e75942. doi:10.1371/journal.pone.0075942 (2013)
  • AKAP79 and PKC, PKA and PDE4, participate in a Gq-linked muscarinic receptor and adenylyl cyclase 2 cAMP signalling complex. JX Shen and DMF Cooper. Biochem. J. 455, 47-56 (2013)


  • A key phosphorylation site in AC8 mediates regulation of Ca2+-dependent cAMP dynamics by an AC8-AKAP79-PKA signalling complex. D Willoughby, ML Halls, KL Everett, A Ciruela P Skroblin, E Klussmann and DMF Cooper. J. Cell Sci. 125, 5850-5859 (2012)
  • Distinct mechanisms of calmodulin binding and regulation of adenylyl cyclases 1 and 8. N Masada, S Schaks, SE Jackson, A Sinz, and DMF Cooper. Biochemistry 51, 7917-7929 (2012)
  • Muscarinic receptors stimulate AC2 by novel phosphorylation sites while G subunits exert opposing effects depending on the G protein source. JX Shen, S Wachten, ML Halls, KL Everett and DMF Cooper. Biochem. J. 447, 393-405 (2012)
  • Direct binding of AC8 and Orai1 underlies dynamic Ca2+-regulated cAMP signalling.  D Willoughby, KL Everett, ML Halls, J Pacheco, P Skroblin, L Vaca, E Klussmann and DMF Cooper Science Signaling (2012) 5, ra29 
  • Adenylyl cyclase AC8 directly controls its micro-environment by recruiting the actin cytoskeleton in a cholesterol-rich milieu. LJ Ayling, SJ Briddon, ML Halls, GRV Hammond, L Vaca, J Pacheco, SJ Hill and DMF Cooper J. Cell Sci. 125 :869-886 (2012)


  • Palmitoylation targets AKAP79 protein to lipid rafts and promotes its regulation of Calcium sensitive adenylyl cyclase type 8. I Delint-Ramirez, D Willoughby, GVR Hammond, LJ Ayling and DMF Cooper J. Biol. Chem. 286: 32962-32975 (2011)
  • Filamin A is a target of membrane AC activity but not cytosolic adenylyl cyclase activity SL Sayner, R Balczon, DW Frank, DMF Cooper, and T Stevens. American Journal of Physiology - Lung Cellular and Molecular Physiology 301: L117-L124 (2011)
  • Role of phosphodiesterase and adenylate cyclase isozymes in murine colonic glucagon-like peptide 1 secreting cells. RS Friedlander, CE Moss, J Mace , HE Parker,G Tolhurst, AM Habib, S Wachten, DMF Cooper, FM Gribble, F Reimann. British Journal of Pharmacology 163, 261-271 (2011)
  • Small molecule AKAP-protein kinase A (PKA) interaction disruptors that activate PKA interfere with compartmentalized cAMP signaling in cardiac myocytes. F Christian,  M Szaszak, S Friedl, S Drewianka, D Lorenz, A Goncalves, J Furkert, C Vargas, P Schmieder, F Goetz, K Zuehlke, M Moutty, H Goettert, M Joshi, B Reif, H Haase, I Morano, S Grossmann, A Klukovits, J Verli, R Gaspar, C Noack, M Bergmann, R Kass, K Hampel, D Kashin, H-G Genieser, FW Herberg, D Willoughby, DMF Cooper, GS Baillie, MD Houslay, JP von Kries, B Zimmermann, W Rosenthal, and E Klussmann. J. Biol. Chem. 286, 9079-9096 (2011)
  • Regulation by Ca2+-signaling pathways of adenylyl cyclases. ML Halls and DMF Cooper. Cold Spring Harbor Perspectives in Biology 3:a004143 (2011)


  • Sub-picomolar relaxin signaling by a pre-assembled RXFP1, AKAP-79, AC2, ß-arrestin 2, PDE4D3 complex. ML Halls and DMF CooperEMBO Journal29, 2772-2787 (2010)
  • A-kinase anchoring protein 79/150 interacts with adenylyl cyclase type 8 and regulates Ca2+-dependent cAMP synthesis in pancreatic and neuronal systems. D Willoughby, N Masada, S Wachten, M Pagano, ML Halls, KL Everett, A Ciruela and DMF Cooper. J. Biol. Chem. 285 20328-20342 (2010)
  • Distinct pools of cAMP centre on different adenylyl cyclase isoforms in pituitary-derived GH3B6 cells. S. Wachten, N Masada, LJ Ayling, A Ciruela, VO Nikolaev, MJ Lohse and DMF Cooper.J Cell Science 123, 95-106 (2010)
  • A-kinase anchoring protein 79/150 interacts with adenylyl cyclase type 8 and regulates Ca2+-dependent cAMP synthesis in pancreatic and neuronal systems. D Willoughby, N Masada, S Wachten, M Pagano, ML Halls, KL Everett, A Ciruela and DMF Cooper. J. Biol. Chem. 285 20328-20342 (2010)


  • Separate Elements within a Single IQ-like Motif in Adenylyl Cyclase Type 8 Impart Ca2+/CaM Binding and Autoinhibition. DA. MacDougall, S Wachten, A Ciruela, A Sinz, and DMF Cooper.J. Biol. Chem. 284 15573-15588 (2009)
  • Structural basis for inhibition of mammalian adenylyl cyclase by Calcium. Mou, TC, Masada, N, Cooper, DMF and Sprang, SS. Biochemistry48, 3387-3397 (2009)
  • Distinct mechanisms of regulation by Ca2+/calmodulin of type 1 and 8 adenylyl cyclases support their different physiological roles. N Masada, A Ciruela, DA MacDougall, and DMF Cooper J. Biol. Chem. 284, 4451-4463 (2009)
  • Insights into the residence in lipid rafts of adenylyl cyclase AC8 and its regulation by capacitative Calcium entry.M Pagano, MA. Clynes, N Masada, A Ciruela, LJ Ayling, S Wachten and DMF Cooper.Am. J. Phys. 296, C607-C619 (2009)
  • Capacitative Ca2+ entry via Orai1 and STIM1 regulates adenylyl cyclase type 8. ACL Martin, D Willoughby, A Ciruela, LJ Ayling M Pagano, S Wachten, A Tengholm and DMF Cooper. Mol. Pharmacol. 75, 830-842 (2009)


  • Live cell imaging of cAMP dynamics. Review. D Willoughby & DMF Cooper Nature Methods 5, 29 -36 (2008)
  • Kinetic properties of Ca2+/Calmodulin-dependent phosphodiesterase isoforms dictate intracellular cAMP dynamics in response to elevation of cytosolic Ca2+ Goraya, TA, Masada, N., Ciruela, A., Willoughby, D., Clynes, MJ and Cooper, DMF Cellular Signalling 20, 359-374 (2008)


  • Organization and Ca2+ regulation of adenylyl cyclases in cAMP microdomains. Willoughby, D and Cooper, DMF. Physiological Reviews 87, 965-1010 (2007)
  • Dynamic regulation, desensitization and crosstalk in discrete sub-cellular microdomains during β2‑adrenoceptor and prostanoid receptor cAMP signalling. Willoughby, D., Baillie, G., Lynch, MJ., Ciruela, A., Houslay, MD and Cooper, DMF. J. Biol. Chem. 282 34235-34249 (2007)
  • Cooper, D. (2007), "Calcium-Regulated Adenylyl Cyclases and Cyclic AMP Compartmentalization", in Simpson, A. (ed.), Calcium Signaling: Regulation, Mechanisms, Effectors, Role in Disease and Recent Advances, The Biomedical & Life Sciences Collection, Henry Stewart Talks Ltd, London 


  • A direct interaction between the N-terminus of adenylyl cyclase AC 8 and the catalytic subunit of protein phosphatase 2A. AJ Crossthwaite, A Ciruela, TF Rayner and DMF Cooper. Mol. Pharmacol. 69, 608-617 (2006)
  • Ca2+ stimulation of adenylyl cyclase generates dynamic oscillations in cyclic AMP. D Willoughby and DMF Cooper. J Cell Science 119, 828-836 (2006)
  • A specific pattern of phosphodiesterases controls the cAMP signals generated by different Gs-coupled receptors in adult rat ventricular myocytes. F Rochais, A Abi-Gerges, K Horner, F Lefebvre, DMF Cooper, M Conti, R Fischmeister and G Vandecasteele. Circulation Research 98, 1081-1088 (2006)
  • Cyclic guanosine monophosphate compartmentation in rat cardiac myocytes. LRV Castro, I Verde, DMF Cooper and R Fischmeister. Circulation 113, 2221-2228 (2006)
  • An anchored PKA and PDE4 complex regulates subplasmalemmal cAMP dynamics. D Willoughby, W Wong, J Schaack, JD Scott and DMF Cooper. EMBO J 25, 2051-2061 (2006)
  • The role of calmodulin recruitment in Ca2+-stimulation of adenylyl cyclase type 8. RE Simpson, A Ciruela and DMF Cooper. J Biol Chem 281, 17379-17389 (2006)
  • Capacitative and OAG-activated Ca2+ entry distinguished using adenylyl cyclase type 8. ACL Martin and DMF Cooper Mol. Pharmacol 70, 769-777 (2006)
  • Higher order organization and regulation of adenylyl cyclases. DMF Cooper and AJ Crossthwaite Trends in Pharmacological Sciences 27, 426-431(2006)


  • The cytosolic domains of Ca2+-sensitive adenylyl cyclases dictate their targeting to plasma membrane lipid rafts. AJ Crossthwaite, T Seebacher, N Masada, A Ciruela, K Dufraux, JE Schultz and DMF Cooper J. Biol. Chem. 280, 6380-6391 (2005)
  • Ca2+/calmodulin-dependent phosphodiesterase (PDE1): Current perspectives. TA Goraya and DMF Cooper Cellular Signalling 17, 789-797 (2005)
  • Localized Na+/H+ Exchanger 1 expression protects Ca2+-regulated adenylyl cyclases from changes in intracellular pH. D Willoughby, N Masada, A Ciruela, AJ Crossthwaite and DMF Cooper. J. Biol. Chem. 280, 30864-30872 (2005)
  • Mapping Protein Interfaces by Chemical Cross-Linking and FTICR Mass Spectrometry: Application to a Calmodulin / Adenylyl Cyclase 8 Peptide Complex. A Schmidt, S Kalkhof, C Ihling, D M F Cooper, and A Sinz. Eur. J. Mass Spectrom. 11, 525-534 (2005)
  • Cooper, DMF and Dessauer, C. Adenylyl cyclase type 6. AfCS-Nature Molecule Pages (2005). (doi:10.1038/mp.a000138.01)


  • Sustained entry of Ca2+ is required to activate Ca2+-calmodulin-dependent phosphodiesterase 1A. TA Goraya, N Masada, A Ciruela and DMF Cooper. J. Biol. Chem. 279, 40494-40504 (2004)
  • Negative feedback exerted by PKA and cAMP phosphodiesterase on subsarcolemmal cAMP signals in intact cardiac myocytes. An in vivo study using adenovirus-mediated expression of CNG channels. F Rochais, G Vandecasteele, F Lefebvre, C Lugnier , H Lum, J-L Mazet, DMF Cooper and R Fischmeister J. Biol. Chem. 279, 52095-52105 (2004)


  • Regulation and organization of adenylyl cyclases and cyclic AMP. DMF Cooper. Biochem. J. 375, 517-529 (2003)


  • Ca2+-sensitive adenylyl cyclase/aequorin chimerae as sensitive probes for discrete modes of elevation of cytosolic Ca2+. DMF Cooper. Methods in Enzymology 345, 105-112 (2002).
  • Dimerization of Mammalian Adenylyl Cyclases; Functional, Biochemical and FRET studies. C Gu, JJ Cali and DMF Cooper. European Journal of Biochemistry 269, 412-421 (2002).
  • Residence of adenylyl cyclase type 8 in caveolae is necessary but not sufficient for regulation by capacitative Ca2+ entry. KE Smith, C Gu, KA Fagan, B Hu and DMF Cooper. J. Biol. Chem. 277, 6025-6031 (2002).
  • Dominant regulation of interendothelial cell gap formation by calcium-inhibited type 6 adenylyl cyclase. D Cioffi, TM Moore, J Schaack, JR Creighton, DMF Cooper and TR Stevens. J. Cell Biology 157,1267-1278 (2002)
  • A critical interplay between Ca2+-inhibition and activation by Mg2+ of AC5 revealed by mutants and chimeric constructs. B Hu, H Nakata, C Gu, T De Beer and DMF Cooper. J. Biol. Chem. 277, 33139-33147 (2002)


  • The two transmembrane clusters of adenylyl cyclase interact tightly to govern the plasma membrane location and function of the enzyme. C. Gu, A. Sorkin and DMF Cooper. Current Biology 11,185-190 (2001).
  • A uniform stimulus triggers distinct cAMP signals in different compartments of a simple cell. TC Rich, KA Fagan, TE Tse, J Schaack, DMF Cooper and J Karpen Proc. Natl. Acad. Sci. USA  98, 13049-13054 (2001).


  • Ca2+, Sr2+ and Ba2+ identify distinct regulatory sites on adenylyl cyclase (AC) types VI and VIII and consolidate the apposition of capacitative cation entry channels and Ca2+-sensitive ACs. C. Gu and D.M.F. Cooper. J. Biol. Chem. 275, 6980-6986 (2000).
  • Sustained endothelial nitric oxide synthase activation requires capacitative Ca2+-entry. S. Lin, K.A. Fagan, K.-X. Li, P.W. Shaul, D.M.F. Cooper and D.M. Rodman J. Biol. Chem. 275,17979-17985 (2000).
  • Characteristics of the Calcium-dependent inhibition of cyclic AMP accumulation by histamine and thaspsigargin in human U373 MG astrocytoma cells. M.P.M. Wong, D.M.F. Cooper, K.W. Young, and J.M. Young. British Journal of Pharmacology 130 1021-1030 (2000)
  • Cyclic nucleotide-gated channels colocalize with adenylyl cyclase in regions of restricted cAMP diffusion. T.C. Rich, K.A. Fagan, H. Nakata, Schaack, J., D.M.F. Cooper and Karpen, J.W. J. Gen. Physiol. 116, 147-161 (2000).
  • Regulation of the Ca2+-inhibitable adenylyl cyclase type VI by capacitative Ca2+-entry requires localization in cholesterol-rich domains. K.A. Fagan, K.E. Smith and D.M.F. Cooper J. Biol. Chem. 275, 26530-26537 (2000)
  • Regulation of a Ca2+-sensitive adenylyl cyclase in an excitable cell; role of voltage-gated versus capacitative Ca2+-entry. KA Fagan, RA Graf, S Tolman, J Schaack and DMF Cooper J. Biol. Chem. 275 40187-40194 (2000).