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Department of Pharmacology



  • Clarke, J.H. & Irvine, R.F. (2012) The activity, evolution and association of phosphatidylinositol 5-phosphate 4-kinases. Adv. Enz. Reg. 52, 40-45.
  • Hammond, G.R.V., Fischer, M.J., Anderson, K.E., Holdich, J., Koteci, A., Balla, T. & Irvine, R.F. (2012) PI4P and PI(4,5)P2 are essential but independent determinants of membrane identity. Science (in press). doi 10.1126/science.1222483


  • Xia, Y., Irvine, R.F. & Giudici, M-L. (2011) Phosphatidylinositol 4-phosphate 5-kinase Iγ_v6, a new splice variant found in rodents and humans. Biochem. Biophys. Res. Commun. 411, 416-420.
  • Irvine, R.F. & Denton, R.M. (2011) Pyro-technic control of metabolism. Science 434, 770-771.


  • Clarke, J.H., Wang, M. & Irvine R.F. (2010) Localization, regulation and function of Type II phosphatidylinositol 5-phosphate 4-kinases. Adv. Enz. Reg. 50, 12-18.
  • Wang, M, Bond, N.J., Letcher, A.J., Richardson, J.P., Lilley, K.S., Irvine, R.F & Clarke, J.H. (2010) Genomic tagging reveals a random association of endogenous PtdIns5P 4-kinases II and II and a partial nuclear localisation of the II isoform. Biochem J. 430, 215-221.
  • Letcher A.J., Schell, M.J. & Irvine R.F. (2010) A femtomole-sensitivity mass assay for inositol hexakisphosphate. In: Barker C.J. (ed). “Inositol phosphates and Lipids: Methods and Protocols”. Methods in Molecular Biology,645, 61-71.


  • Clarke, J.H., Emson, P.C. & Irvine, R.F. (2009) Distribution and neuronal expression of phosphatiylinositol phosphate kinase IIγ in the mouse brain. J. Comp. Neurol. 517, 296-312.
  • Hammond, G.R.V., Schiavo, G. & Irvine, R.F. (2009) Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4P and PtdIns(4,5)P2. Biochem J. 422, 23-35.
  • Hammond, G.R.V., Sim, Y., Lagnado, L. & Irvine, R.F. (2009) Reversible binding and rapid diffusion of proteins in complex with inositol lipids serves to co-ordinate free movement with spatial information. J. Cell Biol. 184, , 297-308.


  • Letcher, A.J., Schell, M.J. & Irvine, R.F. (2008) Do mammals make all their own inositol hexakisphosphate? Biochem. J. 416, 263-270.
  • Clarke J.H., Emson, P.C. & Irvine, R.F. (2008) Localization of phosphatidylinositol phosphate kinase IIγ in kidney to a membrane trafficking compartment within specialized cells of the nephron. Am. J. Physiol. Renal Physiol. 295, F1422-F1430.


  • Richardson, J.P., Wang, M., Clarke, J.H., Patel, K.J. & Irvine. R.F. (2007) Genomic tagging of endogenous Type IIβ phosphatidylinositol 5-phosphate 4-kinase in DT40 cells reveals a nuclear localisation. Cellular Signalling 19, 1309-1314.
  • Lloyd-Burton S.M., Yu, J.C.H., Irvine R.F. & Schell M.J. (2007) Regulation of Ins(1,4,5)P3 3-kinases by calcium and localization in cells. J. Biol. Chem. 282, 9526-9535.


  • Hinchliffe, K.A. & Irvine, R.F. (2006) Regulation of type II PIP kinase by PKD phosphorylation. Cellular Signalling 18,1906-1913.
  • Schell, M.J. & Irvine, R.F. (2006) Calcium-triggered exit of F-actin and IP3 3-kinase A from dendritic spines is rapid and reversible. Eur J. Neurosci. 24, 2491-2503.
  • Veiga, N., Torres, J., Domínguez, S., Mederos, A., Irvine, R.F., Díaz, A., & Kremer, C. (2006) The behaviour of myo-inositol hexakisphosphate in the presence of magnesium(II) and calcium(II): protein-free soluble InsP6 is limited to 50 µM under cytosolic/nuclear conditions. J. Inorg. Biochem. 100, 1800-1810.
  • Giudici M-L., Lee, K., Lim, R. & Irvine, R.F. (2006) The intracellular localisation and mobility of Type Iγ phosphatidylinositol 4P 5-kinase splice variants. FEBS Letters 580, 6933-6937.


  • Powner, D.J., Payne, R.M., Pettitt, T.R., Giudici, M.L., Irvine, R.F. & Wakelam, M.J.O. (2005) Phospholipase D2 stimulates integrin-mediated adhesion via phosphatidylinositol 4-phosphate 5-kinase Iyb. J. Cell Science 118, 2975-2986.
  • Roberts, H.F., Clarke, J.H., Letcher, A.J., Irvine, R.F. & Hinchliffe, K.A. (2005) Effect of lipid kinase expression and cellular stimuli on phosphatidylinositol 5-phosphate levels in mammalian cell lines. FEBS Letters 579, 2868-2872.
  • Brough, D., Sim, Y., Thorn, P. & Irvine, R.F. (2005) The structural integrity of the endoplasmic reticulum, and its possible regulation by inositol 1,3,4,5-tetrakisphosphate. Cell Calcium 38, 153-159.
  • Brough, D., Schell, M.J. & Irvine, R.F. (2005) Agonist-induced regulation of mitochondrial and endoplasmic reticulum motility. Biochem. J. 392, 291-297.
  • Brough, D., Bhatti, F. & Irvine, R.F. (2005) Mobility of proteins associated with the plasma membrane by interaction with inositol lipids. J. Cell Science 118, 3019-3027.


  • Giudici, M.L., Emson, P.C. & Irvine, R.F. (2004) A novel neuronal-specific splice variant of Type I Phosphatidylinositol 4-phosphate 5-kinase isoform gamma. Biochem. J. 379, 489-496.
  • Gonzalez, B., Schell, M.J., Letcher, A.J., Veprintsev, D.B., Irvine, R.F. & Williams, R.J. (2004) The structure of a human inositol 1,4,5-trisphosphate 3-kinase: Interactions in the substrate binding pocket reveal why it is not a phosphoinositide 3-kinase. Mol. Cell 15, 689-701.


  • Clarke, J.H., Letcher, A.J., D'Santos, C.S., Halstead, J.R., Irvine, R.F. & Divecha, N. (2001) Inositol lipids are regulated during cell cycle progression in the nuclei of murine erythroleukaemia cells. Biochem. J. 357, 905-910.
  • Schell, M.J., Erneux, C. & Irvine, R.F. (2001) Inositol 1,4,5-trisphosphate 3-kinase A associates with F-actin and dendritic spines via its N terminus. J. Biol. Chem. 276, 37537-37546.
  • Irvine, R.F. & Schell, M.J. (2001) Back in the water: the return of the inositol phosphates. Nature Revs. Mol. Cell Bio. 2, 327-338.


  • Morris, J.B., Hinchliffe, K.A., Ciruela, A., Letcher, A.J. & Irvine, R.F. (2000) Thrombin stimulation of platelets causes an increase in phosphatidylinositol 5-phosphate revealed by mass assay. FEBS Letters 475, 57-60.
  • Ciruela, A., Hinchliffe, K.A., Divecha, N. & Irvine, R.F. (2000) Nuclear targeting of the beta isoform of Type II Phosphatidylinositol phosphate kinase (Phosphatidylinositol 5-phosphate 4-kinase) by its alpha-helix 7. Biochem J. 346, 587-591.


  • Hinchliffe, K. A., Ciruela, A. & Irvine, R.F. (1998) PIPkins, their substrates and their products: new functions for old enzymes. Biochim. Biophys. Acta 1436, 87-104.