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Publications

 

2018

Sowton AP, Millington-Burgess SL, Murray AJ, Harper MT. Rapid kinetics of changes in oxygen consumption rate in thrombin-stimulated platelets measured by high-resolution respirometry. Biochem Biophys Res Comm, In Press (https://doi.org/10.1016/j.bbrc.2018.08.031)

Wei H, Malcor JM, Harper MT. Lipid rafts are essential for release of phosphatidylserine-exposing extracellular vesicles from platelets. Sci Rep, 8, 9987. 

Davies JE, Apta BHR, Harper MT. Cross-reactivity of HMGB1 antibodies for HMGB2. J Immunol Methods, 456, 72-76.

 

2017

Harper MT. Auranofin, a thioredoxin reductase inhibitor, causes platelet death through reactive oxygen species and calcium overload. Platelets

Taylor KA, Wilson DGS, Harper MT, Pugh N. Extracellular chloride is required for efficient platelet aggregation. Platelets, 29, 79-83. 

 

2016

Metcalfe C, Ramasubramoni A, Pula G, Harper MT, Mundell SJ, Coxon CH. Thioredoxin inhibitors attenuate platelet function and thrombus formation. PLoS One, 11, e0163006.

Williams CM, Savage JS, Harper MT, Moore SF, Hers I, Poole AW. Identification of roles for the SNARE-associated protein, SNAP29, in mouse platelets. Platelets, 27, 286-294.

 

2015

Harper MT, van den Bosch MT, Hers I, Poole AW. Platelet dense granule secretion defects may obscure our understanding of α-granule secretion mechanisms. Blood, 125, 3034-3036.

Williams CM, Harper MT, Goggs R, Walsh TG, Offermanns S, Poole AW. Leukemia-Associated RhoGEF (LARG) is not critical for RhoA regulation, yet is important for platelet activation and thrombosis in mice. J Thromb Haemost, 13, 2102-2107.

Moore SF, Williams CM, Brown E, Blair TA, Harper MT, Coward RJ, Poole AW, Hers I. Loss of the insulin receptor in megakaryocytes/platelets causes thrombocytosis and alterations in autocrine IGF signalling. Cardiovasc Res, 107, 9-19.

Walsh TG, Harper MT, Poole AW. SDF-1α is a novel autocrine activator of platelets operating through its receptor CXCR4. Cell Signal, 27, 37-46.

Golebiewska EM, Harper MT, Williams CM, Savage JS, Goggs R, Fischer von Mollard G, Poole AW. Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability. J Biol Chem, 290, 1536-1545.

 

2014

Williams CM, Harper MT, Poole AW. PKCα negatively regulates in vitro proplatelet formation and in vivo platelet production in mice. Platelets, 25, 62-68.

 

2013

Harper MT, Camacho Londono JE, Quick K, Camacho Londono J, Philipp SE, Birnbaumer L, Freichel M, Poole AW. Transient receptor potential channels function as a coincidence signal detector mediated phosphatidylserine exposure. Science Signalling, 6, ra50.

Harper MT, Poole AW. Chloride channels are necessary for full platelet phosphatidylserine exposure and procoagulant activity. Cell Death Dis, 4, e969.

Harper MT, Savage JS, Poole AW. Comment on “Platelet-derived nucleotides promote tumor cell transendothelial migration and metastasis via P2Y2 receptor” by Schumacher et al. Cancer Cell, 9, 287.

Harper MT, van den Bosch MTJ, Hers I, Poole AW. Absence of phenotype in platelets lacking the motor protein myosin Va. PLoS One, 8, e53239.

Goggs R, Harper MT, Pope RJP, Savage JS, Williams CW, Mundell SJ, Bass M, Mellor H, Poole AW. RhoG regulates platelet granule secretion and thrombus formation in mice. J Biol Chem, 228, 34217-34229.

Moore SF, Hunter RW, Harper MT, Savage JS, Saddiq S, Westbury SK, Poole AW, Mumford AD, Hers I. Dysfunction of PI3 kinase/Rap1b/integrin αIIbβ3 pathway underlies ex vivo platelet hypoactivity in essential thrombocythemia. Blood, 121, 1209-1219.

Savage JS, Williams CM, Konopatskaya O, Hers I, Harper MT, Poole AW. Munc13-4 is critical for thrombosis through regulating release of ADP from platelets. J Thromb Haemost, 11, 771-775.

 

2012

Harper MT, Poole AW. Bcl-xL –inhibitory BH3 mimetic ABT-737 depletes platelet calcium stores. Blood, 119, 4337-4338.

Steele BM, Harper MT, Smolenski AP, Granville K, Poole AW, Fitzgerald DJ, Maguire PM. WNT3a modulates platelet function by regulating small GTPase activity. FEBS Lett, 586, 2267-2272.

 

2011

Harper MT, Poole AW. Contribution of store-operated calcium entry and non-capacitative calcium entry to thrombin-induced calcium signalling in human platelets. Cell Calcium, 50, 351-358.

Harper MT, Poole AW. PKC inhibition enhances Ca2+ signalling and phosphatidylserine exposure downstream of protease-activated receptor-1 but not protease-activated receptor-4 in human platelets. J Thromb Haemost, 9, 1599-1607.

Konopatskaya O, Matthews S, Harper MT, Gilio K, Cosemans JMEM, Williams CM, Navarro N, Carter DA, Heemskerk JWM, Leitges M,  Cantrell D, Poole AW. Protein kinase C mediates platelet secretion and thrombus formation through protein kinase D2. Blood, 118, 416-424.

Heemskerk JW, Harper MT, Cosemans JMEM, Poole AW. Unravelling the different functions of protein kinase C isoforms in platelets. FEBS Lett, 585, 1711-1716.

 

2010

Harper MT, Poole AW. PKCa enhances sodium-calcium exchange during store-operated calcium entry in mouse platelets. Cell Calcium, 48, 333-340.

Gilio K, Harper MT, Cosemanns JMEM, Konopatskaya O,  Munnix ICA, Molkentin JD, Prinzen L, Leigtes M, Heemskerk JWM, Poole AW. Functional divergence of PKC isoforms in thrombus formation on collagen. J Biol Chem, 285, 23410-23419.

Harper MT, Poole AW. PKCq negatively regulates store-independent Ca2+ entry and phosphatidylserine exposure downstream of GPVI in platelets. J Biol Chem, 285, 19865-19873.

Harper MT, Sage SO. Src family tyrosine kinases activate thrombin-induced non-capacitative calcium entry in human platelets. Platelets, 21, 445-450.

Harper MT, Mason MJ, Sage SO, Harper AGS. Phorbol ester-evoked Ca2+ signalling in human platelets is via autocrine activation of P2X1 receptors, not a novel non-capacitative Ca2+ entry. J Thromb Haemost, 8, 1604-1613.

Jones ML, Harper MT, Williams CM, Aitkin EW, Poole AW. RGD-ligand mimetic antagonists of integrin αIIbβ3 paradoxically enhance GPVI-induced human platelet activation. J Thromb Haemost, 8, 567-576.

Harper MT, MacCarthy-Murrogh L, Jones ML, Konopatskaya O, Poole AW. Platelets: their role in atherogenesis and thrombosis in coronary heart disease. (In Atherosclerosis: Molecular and Cellular Mechanisms, ed. George SJ, Johnson J).

Harper MT, Poole AW. Diverse functions of protein kinase C isoforms in platelet activation and thrombus formation. J Thromb Haemost, 8, 454-462.

 

2009

Steele BM, Harper MT, Macaulay IC, Foy M, Habas R, Poole AW, Fitzgerald DJ, Maguire PB. Canonical Wnt signalling negatively regulates human platelet function. Proc Nat’l Acad Sci USA, 106, 19836-19841.

Harper MT, Poole AW. PKCq in platelet activation. Blood, 114, 489-491.

Konopatskaya O, Gilio K, Harper MT, Cosemans J, Karim Z, Whiteheart S, Molkentin J, Verkade P, Heemskerk J, Poole AW.  PKCα regulates granule secretion and thrombus formation in mice.  J Clin Invest, 119, 399-407.

 

2008

Hunter RW, Harper MT, Hers I. The PKB inhibitor Akti-1,2 potentiates PAR-1-mediated platelet function independently of its ability to block PKB. J Thromb Haemost, 6, 1923-1932.

Hall KJ, Gilio K, Harper MT, Cosemans J, Heemskerk JW, Poole AW. Genetic evidence that PKCq negatively regulates platelet activation by GPVI.  PLoS One, 3, e3277.

Harper MT, Sage SO. Is calcium-independent phospholipase A2 required for store-operated calcium entry in human platelets? J Thromb Haemost, 6, 1819-1821.

 

2007

Harper MT, Poole AW. Isoform-specific functions of PKC: the platelet paradigm. Biochem Soc Trans, 35, 1005-1008.

Redondo PC, Harper AGS, Harper MT, Brownlow SL, Rosado JA, Sage SO. TRPC1-associated α-actinin, and not TRPC1 itself, is tyrosine phosphorylated during human platelet activation. J Thromb Haemost, 5, 2476-2483.

 

2006

Harper MT, Sage SO. PAR-1 –dependent pp60src activation is dependent on protein kinase C and increased [Ca2+]i: evidence that pp60src does not regulate PAR-1 –dependent Ca2+ entry in human platelets. J Thromb Haemost, 4, 2695-2703.

Harper MT, Sage SO. The actin cytoskeleton regulates calcium entry activated by PAR-4 but not PAR-1 in human platelets. Platelets, 17 134-142.

Redondo PC, Harper MT, Rosado JA, Sage SO. A role for cofilin in the activation of store-operated calcium entry by de novo conformational coupling in human platelets. Blood, 107, 973-979.

 

2004

Rosado JA, Lopez JJ, Harper AGS, Harper MT, Redondo PC, Pariente JA, Sage SO, Salido GM. Two pathways for store-mediated calcium entry differentially dependent on the actin cytoskeleton in human platelets. J Biol Chem, 278 29231-29235.

Brownlow SL, Harper AGS, Harper MT, Sage SO. A role for hTRPC1 and lipid raft domains in store-mediated calcium entry in human platelets. Cell Calcium, 35 107-113.