Professor Mark Howarth

Mark did graduate work in molecular immunology at Oxford University Institute of Molecular Medicine and Southampton University Cancer Sciences. In his postdoctoral work at MIT he developed tools in chemical biology, advanced microscopy and nanotechnology to understand receptor trafficking. He became Professor of Protein Nanotechnology at Oxford University Department of Biochemistry, receiving the Royal Society of Chemistry Norman Heatley Prize for Chemical Biology. He is a founder of SpyBiotech, which is now running vaccine clinical trials, and several members of his group have founded their own start-up companies. Mark gives lectures and workshops on Entrepreneurship to undergraduates and graduate groups. Resources from his group have been distributed to more than a thousand academic groups and licensed to a range of companies. His work has been funded by ERC, BBSRC, EPSRC, MRC, Wellcome Trust and through collaborations with multiple pharma and biotech companies. He moved to Cambridge to take up the Sheild Chair of Pharmacology in 2022.

Publications

Key publications:

NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins.
Scheu AHA, Lim SYT, Metzner FJ, Mohammed S, Howarth M. Nature Communications 2021 Jan 29;12(1):717.

Gastrobodies are engineered antibody mimetics resilient to pepsin and hydrochloric acid.
Wicke N, Bedford MR, Howarth M. Communications Biology 2021 Aug 11;4(1):960.

Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice.
Cohen AA, Gnanapragasam PNP, Lee YE, Hoffman PR, Ou S, Kakutani LM, Keeffe JR, Wu HJ, Howarth M, West AP, Barnes CO, Nussenzweig MC, Bjorkman PJ. Science 2021 Feb 12;371(6530):735-741.

A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses. Tan TK, Rijal P, Rahikainen R, Keeble AH…Howarth M*, Townsend AR*. Nature Communications 2021 Jan 22;12(1):542. *Corresponding authors

Overcoming Symmetry Mismatch in Vaccine Nanoassembly through Spontaneous Amidation.
Rahikainen R, Rijal P, Tan TK, Wu HJ, Andersson AC, Barrett JR, Bowden TA, Draper SJ, Townsend AR, Howarth M. Angewandte Chemie 2021 Jan 4;60(1):321-330.

Approaching infinite affinity through engineering of peptide-protein interaction.
Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, Hytönen VP*, Howarth M*. PNAS 2019 116:26523-26533. *Corresponding authors

Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox.
Khairil Anuar INA, Banerjee A, Keeble AH, Carella A, Nikov GI, Howarth M. Nature Communications 2019 10:1734.

Publication (Symplectic Elements)

Journal articles

2023 (Accepted for publication)

Howarth, M., Hytonen, V., Rahikainen, R., Vester, S., Deiters, A., Turkki, P. and Janosko, C., 2023 (Accepted for publication). Visible light-induced specific protein reaction delineates early stages of cell adhesion Journal of the American Chemical Society,
Doi: 10.1021/jacs.3c07827

Gristick, HB., Hartweger, H., Loewe, M., van Schooten, J., Ramos, V., Oliveira, TY., Nishimura, Y., Koranda, NS., Wall, A., Yao, K-H., Poston, D., Gazumyan, A., Wiatr, M., Horning, M., Keeffe, JR., Hoffmann, MAG., Yang, Z., Abernathy, ME., Dam, K-MA., Gao, H., Gnanapragasam, PNP., Kakutani, LM., Pavlovitch-Bedzyk, AJ., Seaman, MS., Howarth, M., McGuire, AT., Stamatatos, L., Martin, MA., West, AP., Nussenzweig, MC. and Bjorkman, PJ., 2023 (Accepted for publication). CD4 binding site immunogens elicit heterologous anti-HIV-1 neutralizing antibodies in transgenic and wild-type animals. Science Immunology, v. 8
Doi: http://doi.org/10.1126/sciimmunol.ade6364

2023

Hills, RA., Tan, TK., Cohen, AA., Keeffe, JR., Keeble, AH., Gnanapragasam, PNP., Storm, KN., Hill, ML., Liu, S., Gilbert-Jaramillo, J., Afzal, M., Napier, A., James, WS., Bjorkman, PJ., Townsend, AR. and Howarth, M., 2023. Multiviral Quartet Nanocages Elicit Broad Anti-Coronavirus Responses for Proactive Vaccinology. bioRxiv,
Doi: http://doi.org/10.1101/2023.02.24.529520

Huang, K-YA., Chen, X., Mohapatra, A., Nguyen, HTV., Schimanski, L., Tan, TK., Rijal, P., Vester, SK., Hills, RA., Howarth, M., Keeffe, JR., Cohen, AA., Kakutani, LM., Wu, Y-M., Shahed-Al-Mahmud, M., Chou, Y-C., Bjorkman, PJ., Townsend, AR. and Ma, C., 2023. Structural basis for a conserved neutralization epitope on the receptor-binding domain of SARS-CoV-2. Nat Commun, v. 14
Doi: http://doi.org/10.1038/s41467-023-35949-8

Keeble, AH., Wood, DP. and Howarth, M., 2023. Design and Evolution of Enhanced Peptide-Peptide Ligation for Modular Transglutaminase Assembly. Bioconjug Chem, v. 34
Doi: 10.1021/acs.bioconjchem.3c00122

2022 (No publication date)

Boskovic, F., Zhu, J., Tivony, R., Ohmann, A., Chen, K., Alawami, M., Đorđević, M., Ermann, N., Pereira Dias, J., Fairhead, M., Howarth, M., Baker, S. and Keyser, UF., 2022 (No publication date). Simultaneous identification of viruses and viral variants with programmable DNA nanobait Nature Nanotechnology,

2022

Dicks, MDJ., Rose, LM., Russell, RA., Bowman, LAH., Graham, C., Jimenez-Guardeño, JM., Doores, KJ., Malim, MH., Draper, SJ., Howarth, M. and Biswas, S., 2022. Modular capsid decoration boosts adenovirus vaccine-induced humoral immunity against SARS-CoV-2. Mol Ther, v. 30
Doi: http://doi.org/10.1016/j.ymthe.2022.08.002

Keeble, AH., Yadav, VK., Ferla, MP., Bauer, CC., Chuntharpursat-Bon, E., Huang, J., Bon, RS. and Howarth, M., 2022. DogCatcher allows loop-friendly protein-protein ligation. Cell Chem Biol, v. 29
Doi: http://doi.org/10.1016/j.chembiol.2021.07.005

Hills, RA. and Howarth, M., 2022. Virus-like particles against infectious disease and cancer: guidance for the nano-architect. Curr Opin Biotechnol, v. 73
Doi: http://doi.org/10.1016/j.copbio.2021.09.012

Vester, SK., Rahikainen, R., Khairil Anuar, INA., Hills, RA., Tan, TK. and Howarth, M., 2022. SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly. Nat Commun, v. 13
Doi: http://doi.org/10.1038/s41467-022-31193-8

2021

Rahikainen, R., Rijal, P., Tan, TK., Wu, H-J., Andersson, A-MC., Barrett, JR., Bowden, TA., Draper, SJ., Townsend, AR. and Howarth, M., 2021. Overcoming Symmetry Mismatch in Vaccine Nanoassembly through Spontaneous Amidation. Angew Chem Int Ed Engl, v. 60
Doi: http://doi.org/10.1002/anie.202009663

Tan, TK., Rijal, P., Rahikainen, R., Keeble, AH., Schimanski, L., Hussain, S., Harvey, R., Hayes, JWP., Edwards, JC., McLean, RK., Martini, V., Pedrera, M., Thakur, N., Conceicao, C., Dietrich, I., Shelton, H., Ludi, A., Wilsden, G., Browning, C., Zagrajek, AK., Bialy, D., Bhat, S., Stevenson-Leggett, P., Hollinghurst, P., Tully, M., Moffat, K., Chiu, C., Waters, R., Gray, A., Azhar, M., Mioulet, V., Newman, J., Asfor, AS., Burman, A., Crossley, S., Hammond, JA., Tchilian, E., Charleston, B., Bailey, D., Tuthill, TJ., Graham, SP., Duyvesteyn, HME., Malinauskas, T., Huo, J., Tree, JA., Buttigieg, KR., Owens, RJ., Carroll, MW., Daniels, RS., McCauley, JW., Stuart, DI., Huang, K-YA., Howarth, M. and Townsend, AR., 2021. A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses. Nat Commun, v. 12
Doi: http://doi.org/10.1038/s41467-020-20654-7

Rahikainen, R., Rijal, P., Tan, TK., Wu, H., Andersson, AC., Barrett, JR., Bowden, TA., Draper, SJ., Townsend, AR. and Howarth, M., 2021. Overcoming Symmetry Mismatch in Vaccine Nanoassembly through Spontaneous Amidation Angewandte Chemie, v. 133
Doi: 10.1002/ange.202009663

Wicke, N., Bedford, MR. and Howarth, M., 2021. Gastrobodies are engineered antibody mimetics resilient to pepsin and hydrochloric acid. Commun Biol, v. 4
Doi: http://doi.org/10.1038/s42003-021-02487-2

Cohen, AA., Gnanapragasam, PNP., Lee, YE., Hoffman, PR., Ou, S., Kakutani, LM., Keeffe, JR., Wu, H-J., Howarth, M., West, AP., Barnes, CO., Nussenzweig, MC. and Bjorkman, PJ., 2021. Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice. Science, v. 371
Doi: http://doi.org/10.1126/science.abf6840

Scheu, AHA., Lim, SYT., Metzner, FJ., Mohammed, S. and Howarth, M., 2021. NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins. Nat Commun, v. 12
Doi: http://doi.org/10.1038/s41467-021-20963-5

2020

Keeble, AH. and Howarth, M., 2020. Power to the protein: enhancing and combining activities using the Spy toolbox. Chem Sci, v. 11
Doi: http://doi.org/10.1039/d0sc01878c

Feng, J., Martin-Baniandres, P., Booth, MJ., Veggiani, G., Howarth, M., Bayley, H. and Rodriguez-Larrea, D., 2020. Transmembrane protein rotaxanes reveal kinetic traps in the refolding of translocated substrates. Commun Biol, v. 3
Doi: http://doi.org/10.1038/s42003-020-0840-5

2019

Andersson, A-MC., Buldun, CM., Pattinson, DJ., Draper, SJ. and Howarth, M., 2019. SnoopLigase peptide-peptide conjugation enables modular vaccine assembly. Sci Rep, v. 9
Doi: http://doi.org/10.1038/s41598-019-40985-w

Keeble, AH., Turkki, P., Stokes, S., Khairil Anuar, INA., Rahikainen, R., Hytönen, VP. and Howarth, M., 2019. Approaching infinite affinity through engineering of peptide-protein interaction. Proc Natl Acad Sci U S A, v. 116
Doi: http://doi.org/10.1073/pnas.1909653116

Khairil Anuar, INA., Banerjee, A., Keeble, AH., Carella, A., Nikov, GI. and Howarth, M., 2019. Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox. Nat Commun, v. 10
Doi: http://doi.org/10.1038/s41467-019-09678-w

2018

Bruun, TUJ., Andersson, A-MC., Draper, SJ. and Howarth, M., 2018. Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination. ACS Nano, v. 12
Doi: http://doi.org/10.1021/acsnano.8b02805

Howarth, M., 2018. Spy and Snoop protein superglues for nano-assembly and tickling the immune system New Biotechnology, v. 44
Doi: 10.1016/j.nbt.2018.05.140

Wieduwild, R. and Howarth, M., 2018. Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions. Biomaterials, v. 180
Doi: http://doi.org/10.1016/j.biomaterials.2018.07.020

Brune, KD. and Howarth, M., 2018. New Routes and Opportunities for Modular Construction of Particulate Vaccines: Stick, Click, and Glue. Front Immunol, v. 9
Doi: http://doi.org/10.3389/fimmu.2018.01432

Howarth, M. and Jaramillo, A., 2018. Editorial overview: Nanobiotechnology: Baby steps and giant strides towards molecular mastery. Curr Opin Biotechnol, v. 51
Doi: http://doi.org/10.1016/j.copbio.2018.05.001

Buldun, CM., Jean, JX., Bedford, MR. and Howarth, M., 2018. SnoopLigase Catalyzes Peptide-Peptide Locking and Enables Solid-Phase Conjugate Isolation. J Am Chem Soc, v. 140
Doi: http://doi.org/10.1021/jacs.7b13237

Banerjee, A. and Howarth, M., 2018. Nanoteamwork: covalent protein assembly beyond duets towards protein ensembles and orchestras. Curr Opin Biotechnol, v. 51
Doi: http://doi.org/10.1016/j.copbio.2017.10.006

2017

Keeble, AH., Banerjee, A., Ferla, MP., Reddington, SC., Anuar, INAK. and Howarth, M., 2017. Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics. Angew Chem Int Ed Engl, v. 56
Doi: http://doi.org/10.1002/anie.201707623

Gilbert, C., Howarth, M., Harwood, CR. and Ellis, T., 2017. Extracellular Self-Assembly of Functional and Tunable Protein Conjugates from Bacillus subtilis. ACS Synth Biol, v. 6
Doi: http://doi.org/10.1021/acssynbio.6b00292

Keeble, AH., Banerjee, A., Ferla, MP., Reddington, SC., Anuar, INAK. and Howarth, M., 2017. Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics Angewandte Chemie, v. 129
Doi: 10.1002/ange.201707623

Jacobsen, MT., Fairhead, M., Fogelstrand, P. and Howarth, M., 2017. Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction. Cell Chem Biol, v. 24
Doi: http://doi.org/10.1016/j.chembiol.2017.06.015

Leneghan, DB., Miura, K., Taylor, IJ., Li, Y., Jin, J., Brune, KD., Bachmann, MF., Howarth, M., Long, CA. and Biswas, S., 2017. Nanoassembly routes stimulate conflicting antibody quantity and quality for transmission-blocking malaria vaccines. Sci Rep, v. 7
Doi: http://doi.org/10.1038/s41598-017-03798-3

Howarth, M., 2017. Smart superglue in streptococci? The proof is in the pulling. J Biol Chem, v. 292
Doi: http://doi.org/10.1074/jbc.H117.777466

Brune, KD., Buldun, CM., Li, Y., Taylor, IJ., Brod, F., Biswas, S. and Howarth, M., 2017. Dual Plug-and-Display Synthetic Assembly Using Orthogonal Reactive Proteins for Twin Antigen Immunization. Bioconjug Chem, v. 28
Doi: http://doi.org/10.1021/acs.bioconjchem.7b00174

Dubacheva, GV., Araya-Callis, C., Geert Volbeda, A., Fairhead, M., Codée, J., Howarth, M. and Richter, RP., 2017. Controlling Multivalent Binding through Surface Chemistry: Model Study on Streptavidin. J Am Chem Soc, v. 139
Doi: http://doi.org/10.1021/jacs.7b00540

2016

Schoene, C., Bennett, SP. and Howarth, M., 2016. SpyRing interrogation: analyzing how enzyme resilience can be achieved with phytase and distinct cyclization chemistries. Sci Rep, v. 6
Doi: http://doi.org/10.1038/srep21151

Brune, KD., Leneghan, DB., Brian, IJ., Ishizuka, AS., Bachmann, MF., Draper, SJ., Biswas, S. and Howarth, M., 2016. Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization. Sci Rep, v. 6
Doi: http://doi.org/10.1038/srep19234

Bano, F., Banerji, S., Howarth, M., Jackson, DG. and Richter, RP., 2016. A single molecule assay to probe monovalent and multivalent bonds between hyaluronan and its key leukocyte receptor CD44 under force. Sci Rep, v. 6
Doi: http://doi.org/10.1038/srep34176

Veggiani, G., Nakamura, T., Brenner, MD., Gayet, RV., Yan, J., Robinson, CV. and Howarth, M., 2016. Programmable polyproteams built using twin peptide superglues. Proc Natl Acad Sci U S A, v. 113
Doi: http://doi.org/10.1073/pnas.1519214113

2015

Howarth, M., 2015. Say it with proteins: an alphabet of crystal structures. Nat Struct Mol Biol, v. 22
Doi: http://doi.org/10.1038/nsmb.3011

Reddington, SC. and Howarth, M., 2015. Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher. Curr Opin Chem Biol, v. 29
Doi: http://doi.org/10.1016/j.cbpa.2015.10.002

2014

Schoene, C., Fierer, JO., Bennett, SP. and Howarth, M., 2014. SpyTag/SpyCatcher Cyclization Confers Resilience to Boiling on a Mesophilic Enzyme Angewandte Chemie, v. 126
Doi: 10.1002/ange.201402519

Fairhead, M., Krndija, D., Lowe, ED. and Howarth, M., 2014. Plug-and-play pairing via defined divalent streptavidins. J Mol Biol, v. 426
Doi: http://doi.org/10.1016/j.jmb.2013.09.016

Fairhead, M., Veggiani, G., Lever, M., Yan, J., Mesner, D., Robinson, CV., Dushek, O., van der Merwe, PA. and Howarth, M., 2014. SpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly. J Am Chem Soc, v. 136
Doi: http://doi.org/10.1021/ja505584f

Li, L., Fierer, JO., Rapoport, TA. and Howarth, M., 2014. Structural analysis and optimization of the covalent association between SpyCatcher and a peptide Tag. J Mol Biol, v. 426
Doi: http://doi.org/10.1016/j.jmb.2013.10.021

Fairhead, M., Shen, D., Chan, LKM., Lowe, ED., Donohoe, TJ. and Howarth, M., 2014. Love-Hate ligands for high resolution analysis of strain in ultra-stable protein/small molecule interaction. Bioorg Med Chem, v. 22
Doi: http://doi.org/10.1016/j.bmc.2014.07.029

Fierer, JO., Veggiani, G. and Howarth, M., 2014. SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Proc Natl Acad Sci U S A, v. 111
Doi: http://doi.org/10.1073/pnas.1315776111

Veggiani, G., Zakeri, B. and Howarth, M., 2014. Superglue from bacteria: unbreakable bridges for protein nanotechnology. Trends Biotechnol, v. 32
Doi: http://doi.org/10.1016/j.tibtech.2014.08.001

Schoene, C., Fierer, JO., Bennett, SP. and Howarth, M., 2014. SpyTag/SpyCatcher cyclization confers resilience to boiling on a mesophilic enzyme. Angew Chem Int Ed Engl, v. 53
Doi: http://doi.org/10.1002/anie.201402519

2013

Koner, AL., Krndija, D., Hou, Q., Sherratt, DJ. and Howarth, M., 2013. Hydroxy-terminated conjugated polymer nanoparticles have near-unity bright fraction and reveal cholesterol-dependence of IGF1R nanodomains. ACS Nano, v. 7
Doi: http://doi.org/10.1021/nn3042122

Jain, J., Veggiani, G. and Howarth, M., 2013. Cholesterol loading and ultrastable protein interactions determine the level of tumor marker required for optimal isolation of cancer cells. Cancer Res, v. 73
Doi: http://doi.org/10.1158/0008-5472.CAN-12-2956

2012

Celik, E., Zakeri, B., Howarth, M. and Moy, VT., 2012. An Irreversible Lock to Proteins for Dynamic Force Spectroscopy at the Mammalian Cell Surface Biophysical Journal, v. 102
Doi: 10.1016/j.bpj.2011.11.3897

Zakeri, B., Fierer, JO., Celik, E., Chittock, EC., Schwarz-Linek, U., Moy, VT. and Howarth, M., 2012. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A, v. 109
Doi: http://doi.org/10.1073/pnas.1115485109

Liu, DS., Phipps, WS., Loh, KH., Howarth, M. and Ting, AY., 2012. Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells. ACS Nano, v. 6
Doi: http://doi.org/10.1021/nn304793z

2011 (Published online)

Chivers, CE., Lowe, E. and Howarth, M., 2011 (Published online). How streptavidin's utility in bionanotechnology was increased: pushing the limits of a non‐covalent interaction The FASEB Journal, v. 25
Doi: http://doi.org/10.1096/fasebj.25.1_supplement.568.2

2011

Alakoskela, J-M., Koner, AL., Rudnicka, D., Köhler, K., Howarth, M. and Davis, DM., 2011. Mechanisms for size-dependent protein segregation at immune synapses assessed with molecular rulers. Biophys J, v. 100
Doi: http://doi.org/10.1016/j.bpj.2011.05.013

Chivers, CE., Koner, AL., Lowe, ED. and Howarth, M., 2011. How the biotin-streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer. Biochem J, v. 435
Doi: http://doi.org/10.1042/BJ20101593

Dalchau, N., Phillips, A., Goldstein, LD., Howarth, M., Cardelli, L., Emmott, S., Elliott, T. and Werner, JM., 2011. A peptide filtering relation quantifies MHC class I peptide optimization. PLoS Comput Biol, v. 7
Doi: http://doi.org/10.1371/journal.pcbi.1002144

2010

Zakeri, B. and Howarth, M., 2010. Spontaneous intermolecular amide bond formation between side chains for irreversible peptide targeting. J Am Chem Soc, v. 132
Doi: http://doi.org/10.1021/ja910795a

Crozat, E., Meglio, A., Allemand, J-F., Chivers, CE., Howarth, M., Vénien-Bryan, C., Grainge, I. and Sherratt, DJ., 2010. Separating speed and ability to displace roadblocks during DNA translocation by FtsK. EMBO J, v. 29
Doi: http://doi.org/10.1038/emboj.2010.29

Chivers, CE., Crozat, E., Chu, C., Moy, VT., Sherratt, DJ. and Howarth, M., 2010. A streptavidin variant with slower biotin dissociation and increased mechanostability. Nat Methods, v. 7
Doi: http://doi.org/10.1038/nmeth.1450

Chivers, CE., Crozat, E., Chu, C., Moy, V., Sherratt, D. and Howarth, M., 2010. A molecular car‐crash: a speeding motor hits a new ultra‐stable non‐covalent interaction The FASEB Journal, v. 24
Doi: 10.1096/fasebj.24.1_supplement.lb168

2009

Holm, L., Moody, P. and Howarth, M., 2009. Electrophilic affibodies forming covalent bonds to protein targets. J Biol Chem, v. 284
Doi: http://doi.org/10.1074/jbc.M109.034322

2008

Howarth, M. and Ting, AY., 2008. Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin. Nat Protoc, v. 3
Doi: http://doi.org/10.1038/nprot.2008.20

Liu, W., Howarth, M., Greytak, AB., Zheng, Y., Nocera, DG., Ting, AY. and Bawendi, MG., 2008. Compact biocompatible quantum dots functionalized for cellular imaging. J Am Chem Soc, v. 130
Doi: http://doi.org/10.1021/ja076069p

Howarth, M., Liu, W., Puthenveetil, S., Zheng, Y., Marshall, LF., Schmidt, MM., Wittrup, KD., Bawendi, MG. and Ting, AY., 2008. Monovalent, reduced-size quantum dots for imaging receptors on living cells. Nat Methods, v. 5
Doi: http://doi.org/10.1038/nmeth.1206

Thirdborough, SM., Roddick, JS., Radcliffe, JN., Howarth, M., Stevenson, FK. and Elliott, T., 2008. Tapasin shapes immunodominance hierarchies according to the kinetic stability of peptide-MHC class I complexes. Eur J Immunol, v. 38
Doi: http://doi.org/10.1002/eji.200737832

2006

Howarth, M., Chinnapen, DJ-F., Gerrow, K., Dorrestein, PC., Grandy, MR., Kelleher, NL., El-Husseini, A. and Ting, AY., 2006. A monovalent streptavidin with a single femtomolar biotin binding site. Nat Methods, v. 3
Doi: http://doi.org/10.1038/nmeth861

Howarth, M. and Ting, AY., 2006. Giving cells a new sugar-coating. Nat Chem Biol, v. 2
Doi: http://doi.org/10.1038/nchembio0306-127

2005

Howarth, M., Takao, K., Hayashi, Y. and Ting, AY., 2005. Targeting quantum dots to surface proteins in living cells with biotin ligase. Proc Natl Acad Sci U S A, v. 102
Doi: http://doi.org/10.1073/pnas.0503125102

Chen, I., Howarth, M., Lin, W. and Ting, AY., 2005. Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nat Methods, v. 2
Doi: http://doi.org/10.1038/nmeth735

2004

Howarth, M., Williams, A., Tolstrup, AB. and Elliott, T., 2004. Tapasin enhances MHC class I peptide presentation according to peptide half-life. Proc Natl Acad Sci U S A, v. 101
Doi: http://doi.org/10.1073/pnas.0306294101

Yingyongnarongkul, B-E., Howarth, M., Elliott, T. and Bradley, M., 2004. DNA transfection screening from single beads. J Comb Chem, v. 6
Doi: http://doi.org/10.1021/cc049945t

Howarth, M. and Elliott, T., 2004. The processing of antigens delivered as DNA vaccines. Immunol Rev, v. 199
Doi: http://doi.org/10.1111/j.0105-2896.2004.00141.x

Yingyongnarongkul, B-E., Howarth, M., Elliott, T. and Bradley, M., 2004. Solid-phase synthesis of 89 polyamine-based cationic lipids for DNA delivery to mammalian cells. Chemistry, v. 10
Doi: http://doi.org/10.1002/chem.200305232

2002

Gao, B., Adhikari, R., Howarth, M., Nakamura, K., Gold, MC., Hill, AB., Knee, R., Michalak, M. and Elliott, T., 2002. Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin. Immunity, v. 16
Doi: http://doi.org/10.1016/s1074-7613(01)00260-6

2001

Barber, LD., Howarth, M., Bowness, P. and Elliott, T., 2001. The quantity of naturally processed peptides stably bound by HLA-A*0201 is significantly reduced in the absence of tapasin. Tissue Antigens, v. 58
Doi: http://doi.org/10.1034/j.1399-0039.2001.580604.x

Book chapters

2021

Buldun, CM., Khairil Anuar, INA. and Howarth, M., 2021. SnoopLigase-Mediated Peptide-Peptide Conjugation and Purification.
Doi: http://doi.org/10.1007/978-1-0716-0928-6_2

2019

Keeble, AH. and Howarth, M., 2019. Insider information on successful covalent protein coupling with help from SpyBank.
Doi: http://doi.org/10.1016/bs.mie.2018.12.010

2016

Schoene, C., Bennett, SP. and Howarth, M., 2016. SpyRings Declassified: A Blueprint for Using Isopeptide-Mediated Cyclization to Enhance Enzyme Thermal Resilience.
Doi: http://doi.org/10.1016/bs.mie.2016.05.004

2015

Fairhead, M. and Howarth, M., 2015. Site-specific biotinylation of purified proteins using BirA.
Doi: http://doi.org/10.1007/978-1-4939-2272-7_12

Sheild Professor of Pharmacology

Contact Details

+44 (0) 1223 3 34021 / Lab 3 34177

https://www.phar.cam.ac.uk/research/Howarth

Not available for consultancy

Affiliations

Classifications:

Academic Staff

Person keywords:

Protein engineering, synthetic biology, cancer signalling, antibodies, vaccines, protein design, protein-protein interactions, immunology, bioconjugation, gastroenterology, directed evolution.

Publications

Publication (Symplectic Elements)

2023 (Accepted for publication)

2023

2022 (No publication date)

2022

2021

2020

2019

2018

2017

2016

2015

2014

2013

2012

2011 (Published online)

2011

2010

2009

2008

2006

2005

2004

2002

2001

2021

2019

2016

2015

Contact Details

Affiliations

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Department

School of Biological Sciences

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