Professor Graham Ladds

Graham studied Biochemistry at the University of Birmingham before completing a PhD in yeast pheromone signalling at Warwick. He continued to work at Warwick as a post-doc studying pro-hormone convertases before securing a 5-year independent fellowship funded through the NHS. This project enabled him to return to his interest of GPCRs. He progressed through the ranks at Warwick become an Associate Professor before leaving in 2015 to join the Department of Pharmacology at Cambridge, where he is also a Fellow of St John’s College. In 2020, he was promoted to a Readership in Receptor Pharmacology and was elected a Fellow of the British Pharmacological Society. His research group use a combination of pharmacological investigations and mathematical modelling to study factors that control agonist bias at GPCRs. These investigations have enabled him to foster strong collaborations with the pharmaceutical industry (GSK, Takeda and Firmenich) which have recently been enhanced though him being awarded a Royal Society Industry Fellowship to collaborate with AstraZeneca.

Publications

Key publications:

1. Shaw, W.M., Yamauchi, H., Mead, J., Gowers, G.F., Oling, D., Larsson, N., Wigglesworth, M.*, Ladds, G.* and Ellis, T.* (2019) Engineering a model cell for rational tuning of GPCR signalling Cell pii: S0092-8674(19)30205-3. doi: 10.1016/j.cell.2019.02.023.

2. Bridge, L., Mead, J., Frattini, E., Winfield, I., and Ladds, G.(2018) Modelling and simulation of biased agonism dynamics at a G protein-coupled receptor. J. Theoretical. Biol.7: 44-65.

3. Weston, C., Winfield, I., Harris, M., Hodgson, R., Shah, A., Dowell, S.J., Moarec, J-C, Woodlock, D.A., Reynolds, C.A., Poyner, D.R., Watkins, H.A. and Ladds, G. (2016) RAMP-directed G protein signaling specificity for the calcitonin gene-related peptide family of receptors. J. Biol. Chem.91: 21925 .

4. Knight, A., Hemmings, J.L., Frenguelli, B.G., Dowell, S.J., Lochner, M. and Ladds, G. (2016) Discovery of Novel Adenosine Receptor Agonists that Exhibit Subtype Selectivity. J. Med Chem. 59: 947.

5. Weston, C., Li, J., Li, N., Barkan, G., Richards, G.O., Roberts, D., Skerry, T.M., Poyner, D., Pardamwar, M., Reynolds, C.A., Dowell, S., Willars, G. and Ladds, G. (2015) Modulation of glucagon receptor pharmacology by RAMP2. J. Biol. Chem. 290: 23009.

Investigator Biography

Graham studied Biochemistry at the University of Birmingham before completing a PhD in yeast pheromone signalling with John Davey at Warwick. He continued to work at Warwick as a post-doc studying pro-hormone convertases before securing a 5-year independent fellowship funded through the NHS. This project enabled him to return to his interest of GPCRs. He was appointed a lecturer at Warwick in 2006. In 2015 he was appointed to a lectureship in the Pharmacology Department, the University of Cambridge. He is a full member of the British Pharmacology Society and regularly attends meetings both in the UK and Australia. He has a long-standing collaboration with Dr Simon Dowell at GlaxoSmithKline. His group is interested in RAMP-mediated signalling bias of family B GPCRs.

Publication (Symplectic Elements)

Theses / dissertations

2023 (No publication date)

Pearce, A., 2023 (No publication date). Influence of Peptide Allosteric Modulators on Agonist Bias at Class B1 G Protein-Coupled Receptors

2023

Hilšer, A., 2023. Drug discovery at class A and class B GPCRs

2022 (No publication date)

Carvalho, S., 2022 (No publication date). Investigating the molecular pharmacology of class A and B G protein-coupled receptors

2021 (No publication date)

Yeung, HY., 2021 (No publication date). Characterisation and pharmacological regulation of GLP-1-mediated glucose homeostasis

Clark, A., 2021 (No publication date). CGRP family of G protein-coupled receptors and their signalling in human cardiovascular cells

Safitri, D., 2021 (No publication date). Modulation of cyclic adenosine monophosphate (cAMP) signalling and its therapeutic potential: pharmacological characterisation of PDE inhibitors

Journal articles

2023 (Accepted for publication)

Ladds, G., Pearce, A., Harris, M. and Yeung, H., 2023 (Accepted for publication). GLP‐1 metabolite GLP‐1(9–36) is a systemic inhibitor of mouse and human pancreatic islet glucagon secretion Diabetologia,
Doi: http://doi.org/10.1007/s00125-023-06060-w

2023

Pan, D., Ladds, G., Rahman, KM. and Pitchford, SC., 2023. Exploring bias in platelet P2Y1 signalling: Host defence versus haemostasis. Br J Pharmacol,
Doi: 10.1111/bph.16191

McElroy, CR., Kopanitsa, L., Helmes, R., Fan, J., Attard, TM., Simister, R., van den Burg, S., Ladds, G., Bailey, DS. and Gomez, LD., 2023. Integrated biorefinery approach to valorise Saccharina latissima biomass: Combined sustainable processing to produce biologically active fucoxanthin, mannitol, fucoidans and alginates Environmental Technology and Innovation, v. 29
Doi: http://doi.org/10.1016/j.eti.2023.103014

2022

Pearce, A., Redfern-Nichols, T., Harris, M., Poyner, DR., Wigglesworth, M. and Ladds, G., 2022. Determining the Effects of Differential Expression of GRKs and β-arrestins on CLR-RAMP Agonist Bias. Front Physiol, v. 13
Doi: http://doi.org/10.3389/fphys.2022.840763

Suchankova, A., Stampelou, M., Koutsouki, K., Pousias, A., Dhingra, L., Barkan, K., Pouli, N., Marakos, P., Tenta, R., Kolocouris, A., Lougiakis, N. and Ladds, G., 2022. Discovery of a High Affinity Adenosine A1/A3 Receptor Antagonist with a Novel 7-Amino-pyrazolo[3,4-d]pyridazine Scaffold. ACS Med Chem Lett, v. 13
Doi: http://doi.org/10.1021/acsmedchemlett.2c00052

Wall, MJ., Hill, E., Huckstepp, R., Barkan, K., Deganutti, G., Leuenberger, M., Preti, B., Winfield, I., Carvalho, S., Suchankova, A., Wei, H., Safitri, D., Huang, X., Imlach, W., La Mache, C., Dean, E., Hume, C., Hayward, S., Oliver, J., Zhao, F-Y., Spanswick, D., Reynolds, CA., Lochner, M., Ladds, G. and Frenguelli, BG., 2022. Selective activation of Gαob by an adenosine A1 receptor agonist elicits analgesia without cardiorespiratory depression. Nat Commun, v. 13
Doi: http://doi.org/10.1038/s41467-022-31652-2

Shaw, WM., Zhang, Y., Lu, X., Khalil, AS., Ladds, G., Luo, X. and Ellis, T., 2022. Screening microbially produced Δ9-tetrahydrocannabinol using a yeast biosensor workflow. Nat Commun, v. 13
Doi: http://doi.org/10.1038/s41467-022-33207-x

Stampelou, M., Suchankova, A., Tzortzini, E., Dhingra, L., Barkan, K., Lougiakis, N., Marakos, P., Pouli, N., Ladds, G. and Kolocouris, A., 2022. Dual A1/A3 Adenosine Receptor Antagonists: Binding Kinetics and Structure-Activity Relationship Studies Using Mutagenesis and Alchemical Binding Free Energy Calculations. J Med Chem, v. 65
Doi: http://doi.org/10.1021/acs.jmedchem.2c01123

Preti, B., Suchankova, A., Deganutti, G., Leuenberger, M., Barkan, K., Manulak, I., Huang, X., Carvalho, S., Ladds, G. and Lochner, M., 2022. Discovery and Structure-Activity Relationship Studies of Novel Adenosine A1 Receptor-Selective Agonists. J Med Chem, v. 65
Doi: http://doi.org/10.1021/acs.jmedchem.2c01414

2021 (Accepted for publication)

Deganutti, G., Barkan, K., Preti, B., Leuenberger, M., Wall, M., Frenguelli, BG., Lochner, M., Ladds, G. and Reynolds, CA., 2021 (Accepted for publication). Deciphering the Agonist Binding Mechanism to the Adenosine A1 Receptor ACS Pharmacology & Translational Science,
Doi: http://doi.org/10.1021/acsptsci.0c00195

Ladds, G., clark, A., Mullooly, N., Safitri, D., Harris, M., de Vries, T., MaassenVanDenBrink, A., Poyner, D., Gianni, D. and Wigglesworth, M., 2021 (Accepted for publication). CGRP, adrenomedullin and adrenomedullin 2 display endogenous GPCR agonist bias in primary human cardiovascular cells Communications Biology,

2021

Kalash, L., Winfield, I., Safitri, D., Bermudez, M., Carvalho, S., Glen, R., Ladds, G. and Bender, A., 2021. Structure-based identification of dual ligands at the A2AR and PDE10A with anti-proliferative effects in lung cancer cell-lines. J Cheminform, v. 13
Doi: http://doi.org/10.1186/s13321-021-00492-5

Deganutti, G., Barkan, K., Ladds, G. and Reynolds, CA., 2021. Multisite Model of Allosterism for the Adenosine A1 Receptor. J Chem Inf Model, v. 61
Doi: http://doi.org/10.1021/acs.jcim.0c01331

Dolezalova, N., Gruszczyk, A., Barkan, K., Gamble, JA., Galvin, S., Moreth, T., O'Holleran, K., Mahbubani, KT., Higgins, JA., Gribble, FM., Reimann, F., Surmacki, J., Andrews, S., Casey, JJ., Pampaloni, F., Murphy, MP., Ladds, G., Slater, NKH. and Saeb-Parsy, K., 2021. Accelerating cryoprotectant diffusion kinetics improves cryopreservation of pancreatic islets. Sci Rep, v. 11
Doi: http://doi.org/10.1038/s41598-021-89853-6

Rosa, M., Noel, T., Harris, M. and Ladds, G., 2021. Emerging roles of adhesion G protein-coupled receptors. Biochem Soc Trans, v. 49
Doi: http://doi.org/10.1042/BST20201144

Shapovalov, V., Kopanitsa, L., Pruteanu, L-L., Ladds, G. and Bailey, DS., 2021. Transcriptomics-Based Phenotypic Screening Supports Drug Discovery in Human Glioblastoma Cells. Cancers (Basel), v. 13
Doi: http://doi.org/10.3390/cancers13153780

Kopanitsa, L., Kopanitsa, MV., Safitri, D., Ladds, G. and Bailey, DS., 2021. Suppression of Proliferation of Human Glioblastoma Cells by Combined Phosphodiesterase and Multidrug Resistance-Associated Protein 1 Inhibition. Int J Mol Sci, v. 22
Doi: http://doi.org/10.3390/ijms22189665

Winfield, I., Barkan, K., Routledge, S., Robertson, NJ., Harris, M., Jazayeri, A., Simms, J., Reynolds, CA., Poyner, DR. and Ladds, G., 2021. The Role of ICL1 and H8 in Class B1 GPCRs; Implications for Receptor Activation. Front Endocrinol (Lausanne), v. 12
Doi: http://doi.org/10.3389/fendo.2021.792912

Ladds, G., Carvalho, S. and Pearce, A., 2021. Novel mathematical and computational models of G protein-coupled receptor signalling Current Opinion in Endocrine and Metabolic Research, v. 16
Doi: http://doi.org/10.1016/j.coemr.2020.07.002

2020 (Accepted for publication)

Barkan, K., Lagarias, P., Stampelou, M., Stamatis, D., Hoare, S., Safitri, D., Klotz, K-N., Vrontaki, E., Ladds, G. and Kolocouris, A., 2020 (Accepted for publication). Pharmacological Characterisation of Novel Adenosine A3 Receptor Antagonists Scientific Reports,

2020

Clark, A., Mullooly, N., Safitri, D., Harris, M., de Vries, T., Van Den Brink, AM., Poyner, D., Giani, D., Wigglesworth, M. and Ladds, G., 2020. CGRP-receptor family reveals endogenous GPCR agonist bias and its significance in primary human cardiovascular cells
Doi: http://doi.org/10.1101/2020.12.21.423730

Routledge, SJ., Simms, J., Clark, A., Yeung, HY., Wigglesworth, MJ., Dickerson, IM., Kitchen, P., Ladds, G. and Poyner, DR., 2020. Receptor component protein, an endogenous allosteric modulator of family B G protein coupled receptors. Biochim Biophys Acta Biomembr, v. 1862
Doi: http://doi.org/10.1016/j.bbamem.2019.183174

Safitri, D., Harris, M., Potter, H., Yan Yeung, H., Winfield, I., Kopanitsa, L., Svensson, F., Rahman, T., Harper, MT., Bailey, D. and Ladds, G., 2020. Elevated intracellular cAMP concentration mediates growth suppression in glioma cells. Biochem Pharmacol, v. 174
Doi: http://doi.org/10.1016/j.bcp.2020.113823

Lagarias, P., Barkan, K., Tzortzini, E., Stampelou, M., Vrontaki, E., Ladds, G. and Kolocouris, A., 2020. Correction to Insights to the Binding of a Selective Adenosine A3 Receptor Antagonist using Molecular Dynamic Simulations, MM-PBSA and MM-GBSA Free Energy Calculations, and Mutagenesis. J Chem Inf Model, v. 60
Doi: http://doi.org/10.1021/acs.jcim.0c00240

Greenhalgh, JC., Chandran, A., Harper, MT., Ladds, G. and Rahman, T., 2020. Proposed model of the Dictyostelium cAMP receptors bound to cAMP. J Mol Graph Model, v. 100
Doi: http://doi.org/10.1016/j.jmgm.2020.107662

Knight, A., Hemmings, JL., Winfield, I., Leuenberger, M., Frattini, E., Frenguelli, BG., Dowell, SJ., Lochner, M. and Ladds, G., 2020. Correction to Discovery of Novel Adenosine Receptor Agonists That Exhibit Subtype Selectivity. J Med Chem, v. 63
Doi: http://doi.org/10.1021/acs.jmedchem.0c01416

Marti-Solano, M., Crilly, SE., Malinverni, D., Munk, C., Harris, M., Pearce, A., Quon, T., Mackenzie, AE., Wang, X., Peng, J., Tobin, AB., Ladds, G., Milligan, G., Gloriam, DE., Puthenveedu, MA. and Babu, MM., 2020. Combinatorial expression of GPCR isoforms affects signalling and drug responses. Nature, v. 587
Doi: http://doi.org/10.1038/s41586-020-2888-2

Deganutti, G., Barkan, K., Preti, B., Leuenberger, M., Wall, M., Frenguelli, B., Lochner, M., Ladds, G. and Reynolds, C., 2020. Deciphering the Agonist Binding Mechanism to the Adenosine A1 Receptor
Doi: http://doi.org/10.1101/2020.10.22.350827

Deganutti, G., Barkan, K., Ladds, G. and Reynolds, C., 2020. A Multisite Model of Allosterism for the Adenosine A1 Receptor
Doi: http://doi.org/10.1101/2020.10.14.338822

Marti-Solano, M., Crilly, SE., Malinverni, D., Munk, C., Harris, M., Pearce, A., Quon, T., Mackenzie, AE., Wang, X., Peng, J., Tobin, AB., Ladds, G., Milligan, G., Gloriam, DE., Puthenveedu, MA. and Babu, MM., 2020. Author Correction: Combinatorial expression of GPCR isoforms affects signalling and drug responses. Nature, v. 588
Doi: http://doi.org/10.1038/s41586-020-2999-9

2019 (Accepted for publication)

Bailey, S., Harris, M., Barkan, K., Winfield, I., Harper, MT., Simms, J., Ladds, G., Wheatley, M. and Poyner, D., 2019 (Accepted for publication). Interactions between RAMP2 and CRF receptors: The effect of receptor subtypes, splice variants and cell context Biochimica et Biophysica Acta (BBA) - Biomembranes,
Doi: http://doi.org/10.1016/j.bbamem.2019.02.008

Stamatis, D., Lagarias, P., Barkan, K., Vrontaki, E., Ladds, G. and Kolocouris, A., 2019 (Accepted for publication). Structural Characterization of Agonist Binding to an A3 Adenosine Receptor through Biomolecular Simulations and Mutagenesis Experiments. Journal of Medicinal Chemistry,
Doi: http://doi.org/10.1021/acs.jmedchem.9b01164

2019

Neganova, I., Cotts, L., Banks, P., Gassner, K., Shukurov, A., Armstrong, L., Ladds, G. and Lako, M., 2019. Endothelial Differentiation G Protein-Coupled Receptor 5 Plays an Important Role in Induction and Maintenance of Pluripotency. Stem Cells, v. 37
Doi: http://doi.org/10.1002/stem.2954

Shaw, W., Yamauchi, H., Gowers, G-O., Bell, D., Oling, NL., Wigglesworth, M., Ladds, G. and Ellis, T., 2019. Engineering a Model Cell for Rational Tuning of GPCR Signaling Cell,
Doi: http://doi.org/10.1016/j.cell.2019.02.023

Greenhalgh, JC., Chandran, A., Harper, MT., Ladds, G. and Rahman, T., 2019. Proposed model of the Dictyostelium cAMP receptors bound to cAMP
Doi: http://doi.org/10.1101/694810

Barkan, K., Lagarias, P., Stampelou, M., Stamatis, D., Hoare, S., Klotz, K-N., Vrontaki, E., Kolocouris, A. and Ladds, G., 2019. Pharmacological Characterisation of Novel Adenosine Receptor A3R Antagonists
Doi: http://doi.org/10.1101/693796

Mackie, DI., Nielsen, NR., Harris, M., Singh, S., Davis, RB., Dy, D., Ladds, G. and Caron, KM., 2019. RAMP3 determines rapid recycling of atypical chemokine receptor-3 for guided angiogenesis. Proc Natl Acad Sci U S A, v. 116
Doi: 10.1073/pnas.1905561116

Lagarias, P., Barkan, K., Tzortzini, E., Stampelou, M., Vrontaki, E., Ladds, G. and Kolocouris, A., 2019. Insights to the Binding of a Selective Adenosine A3 Receptor Antagonist Using Molecular Dynamic Simulations, MM-PBSA and MM-GBSA Free Energy Calculations, and Mutagenesis. J Chem Inf Model, v. 59
Doi: http://doi.org/10.1021/acs.jcim.9b00751

Recino, A., Barkan, K., Schmidt-Christensen, A., Nilsson, J., Holmes, N., Howie, D., Holmberg, D., Larsson, P., Flodström-Tullberg, M., Laraia, L., Spring, DR., Hecksher-Sørensen, J., Cooke, A., Ladds, G. and Wållberg, M., 2019. GLP-1R is downregulated in beta cells of NOD mice and T1D patients
Doi: 10.1101/845776

2018 (Accepted for publication)

Khaled, WT., Lazarus, K., Hadi, F., Zambon, E., Bach, K., Santolla, M., Watson, J., Correia, L., Das, M., Ugur, R., Pensa, S., Becker, L., Campos, L., Ladds, G., Liu, P., Evan, G., McCaughan, F., Lee, J., Calado, D. and Le Quesne, J., 2018 (Accepted for publication). BCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous carcinoma Nature Communications,

Harris, M., Svensson, F., Kopanitsa, L., Ladds, GR. and Bailey, D., 2018 (Accepted for publication). Emerging Patents in the Therapeutic Areas of Glioma and Glioblastoma. Expert Opinion on Therapeutic Patents,
Doi: http://doi.org/10.1080/13543776.2018.1494155.

Bridge, L., Mead, J., Frattini, E., Winfield, I. and Ladds, GR., 2018 (Accepted for publication). Modelling and simulation of biased agonism dynamics at a G protein-coupled receptor Journal of Theoretical Biology,

2018

Harris, M., Svensson, F., Kopanitsa, L., Ladds, G. and Bailey, D., 2018. Emerging patents in the therapeutic areas of glioma and glioblastoma. Expert Opin Ther Pat, v. 28
Doi: http://doi.org/10.1080/13543776.2018.1494155

Shaw, W., Yamauchi, H., Mead, J., Gowers, G-O., Öling, D., Larsson, N., Wigglesworth, M., Ladds, G. and Ellis, T., 2018. Engineering a model cell for rational tuning of GPCR signaling
Doi: http://doi.org/10.1101/390559

2017 (Accepted for publication)

Kalash, L., 2017 (Accepted for publication). Computer-aided design of multi-target ligands at A1R, A2AR and PDE10A, key proteins in neurodegenerative diseases Journal of Cheminformatics,
Doi: http://doi.org/10.1186/s13321-017-0249-4

2017

Routledge, SJ., Ladds, G. and Poyner, DR., 2017. The effects of RAMPs upon cell signalling. Mol Cell Endocrinol, v. 449
Doi: http://doi.org/10.1016/j.mce.2017.03.033

Recino, A., Barkan, K., Ladds, G., Cooke, A., Wong, FS. and Wallberg, M., 2017. Hyperglycemia does not affect antigen specific activation and cytolytic killing by CD8+ T cells in vivo Bioscience Reports, v. 37
Doi: http://doi.org/10.1042/bsr20171079

2016

Weston, C., Winfield, I., Harris, M., Hodgson, R., Shah, A., Dowell, SJ., Mobarec, JC., Woodcock, DA., Reynolds, CA., Poyner, DR., Watkins, HA. and Ladds, G., 2016. Receptor activity-modifying protein-directed G protein signaling specificity for the calcitonin gene-related peptide family of receptors. J Biol Chem, v. 291
Doi: http://doi.org/10.1074/jbc.A116.751362

Knight, A., Hemmings, JL., Winfield, I., Leuenberger, M., Frattini, E., Frenguelli, BG., Dowell, SJ., Lochner, M. and Ladds, G., 2016. Discovery of Novel Adenosine Receptor Agonists That Exhibit Subtype Selectivity. J Med Chem, v. 59
Doi: http://doi.org/10.1021/acs.jmedchem.5b01402

Hennig, A., Markwart, R., Wolff, K., Schubert, K., Cui, Y., Prior, IA., Esparza-Franco, MA., Ladds, G. and Rubio, I., 2016. Feedback activation of neurofibromin terminates growth factor-induced Ras activation. Cell Commun Signal, v. 14
Doi: http://doi.org/10.1186/s12964-016-0128-z

Hay, DL., Walker, CS., Gingell, JJ., Ladds, G., Reynolds, CA. and Poyner, DR., 2016. Receptor activity-modifying proteins; multifunctional G protein-coupled receptor accessory proteins. Biochem Soc Trans, v. 44
Doi: http://doi.org/10.1042/BST20150237

Weston, C., Winfield, I., Harris, M., Hodgson, R., Shah, A., Dowell, SJ., Mobarec, JC., Woodlock, DA., Reynolds, CA., Poyner, DR., Watkins, HA. and Ladds, G., 2016. Receptor Activity-modifying Protein-directed G Protein Signaling Specificity for the Calcitonin Gene-related Peptide Family of Receptors. J Biol Chem, v. 291
Doi: http://doi.org/10.1074/jbc.M116.751362

2015

Hennig, A., Markwart, R., Esparza-Franco, MA., Ladds, G. and Rubio, I., 2015. Ras activation revisited: role of GEF and GAP systems. Biol Chem, v. 396
Doi: http://doi.org/10.1515/hsz-2014-0257

Lockley, R., Ladds, G. and Bretschneider, T., 2015. Image based validation of dynamical models for cell reorientation. Cytometry A, v. 87
Doi: http://doi.org/10.1002/cyto.a.22600

Weston, C., Lu, J., Li, N., Barkan, K., Richards, GO., Roberts, DJ., Skerry, TM., Poyner, D., Pardamwar, M., Reynolds, CA., Dowell, SJ., Willars, GB. and Ladds, G., 2015. Modulation of Glucagon Receptor Pharmacology by Receptor Activity-modifying Protein-2 (RAMP2). J Biol Chem, v. 290
Doi: http://doi.org/10.1074/jbc.M114.624601

Prokic, EJ., Weston, C., Yamawaki, N., Hall, SD., Jones, RSG., Stanford, IM., Ladds, G. and Woodhall, GL., 2015. Cortical oscillatory dynamics and benzodiazepine-site modulation of tonic inhibition in fast spiking interneurons. Neuropharmacology, v. 95
Doi: http://doi.org/10.1016/j.neuropharm.2015.03.006

Shigeto, M., Ramracheya, R., Tarasov, AI., Cha, CY., Chibalina, MV., Hastoy, B., Philippaert, K., Reinbothe, T., Rorsman, N., Salehi, A., Sones, WR., Vergari, E., Weston, C., Gorelik, J., Katsura, M., Nikolaev, VO., Vennekens, R., Zaccolo, M., Galione, A., Johnson, PRV., Kaku, K., Ladds, G. and Rorsman, P., 2015. GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation. J Clin Invest, v. 125
Doi: http://doi.org/10.1172/JCI81975

Croft, W., Elliott, CM., Ladds, G., Stinner, B., Venkataraman, C. and Weston, C., 2015. Parameter identification problems in the modelling of cell motility. J Math Biol, v. 71
Doi: http://doi.org/10.1007/s00285-014-0823-6

2014

Weston, C., Poyner, D., Patel, V., Dowell, S. and Ladds, G., 2014. Investigating G protein signalling bias at the glucagon-like peptide-1 receptor in yeast. Br J Pharmacol, v. 171
Doi: http://doi.org/10.1111/bph.12716

Lock, A., Forfar, R., Weston, C., Bowsher, L., Upton, GJG., Reynolds, CA., Ladds, G. and Dixon, AM., 2014. One motif to bind them: A small-XXX-small motif affects transmembrane domain 1 oligomerization, function, localization, and cross-talk between two yeast GPCRs. Biochim Biophys Acta, v. 1838
Doi: http://doi.org/10.1016/j.bbamem.2014.08.019

2013

Croft, W., Hill, C., McCann, E., Bond, M., Esparza-Franco, M., Bennett, J., Rand, D., Davey, J. and Ladds, G., 2013. A physiologically required G protein-coupled receptor (GPCR)-regulator of G protein signaling (RGS) interaction that compartmentalizes RGS activity. J Biol Chem, v. 288
Doi: http://doi.org/10.1074/jbc.M113.497826

Mos, M., Esparza-Franco, MA., Godfrey, EL., Richardson, K., Davey, J. and Ladds, G., 2013. The role of the RACK1 ortholog Cpc2p in modulating pheromone-induced cell cycle arrest in fission yeast. PLoS One, v. 8
Doi: http://doi.org/10.1371/journal.pone.0065927

Weston, C., Bond, M., Croft, W. and Ladds, G., 2013. The coordination of cell growth during fission yeast mating requires Ras1-GTP hydrolysis. PLoS One, v. 8
Doi: http://doi.org/10.1371/journal.pone.0077487

Bond, M., Croft, W., Tyson, R., Bretschneider, T., Davey, J. and Ladds, G., 2013. Quantitative analysis of human ras localization and function in the fission yeast Schizosaccharomyces pombe. Yeast, v. 30
Doi: http://doi.org/10.1002/yea.2949

2011

Davey, J. and Ladds, G., 2011. Heterologous expression of GPCRs in fission yeast. Methods Mol Biol, v. 746
Doi: http://doi.org/10.1007/978-1-61779-126-0_7

2010

Li, S., Spooner, RA., Allen, SCH., Guise, CP., Ladds, G., Schnöder, T., Schmitt, MJ., Lord, JM. and Roberts, LM., 2010. Folding-competent and folding-defective forms of ricin A chain have different fates after retrotranslocation from the endoplasmic reticulum. Mol Biol Cell, v. 21
Doi: http://doi.org/10.1091/mbc.e09-08-0743

2009

Hill, C., Goddard, A., Ladds, G. and Davey, J., 2009. The cationic region of Rhes mediates its interactions with specific Gbeta subunits. Cell Physiol Biochem, v. 23
Doi: http://doi.org/10.1159/000204075

Ladds, G., Zervou, S., Vatish, M., Thornton, S. and Davey, J., 2009. Regulators of G protein signalling proteins in the human myometrium. Eur J Pharmacol, v. 610
Doi: http://doi.org/10.1016/j.ejphar.2009.03.042

Smith, B., Hill, C., Godfrey, EL., Rand, D., van den Berg, H., Thornton, S., Hodgkin, M., Davey, J. and Ladds, G., 2009. Dual positive and negative regulation of GPCR signaling by GTP hydrolysis. Cell Signal, v. 21
Doi: http://doi.org/10.1016/j.cellsig.2009.03.004

2008

Wright, MH., Farquhar, MJ., Aletrari, M-O., Ladds, G. and Hodgkin, MN., 2008. Identification of caspase 3 motifs and critical aspartate residues in human phospholipase D1b and phospholipase D2a. Biochem Biophys Res Commun, v. 369
Doi: http://doi.org/10.1016/j.bbrc.2008.02.064

Hill, C., Brownlie, Z., Davey, J., Milligan, G. and Ladds, G., 2008. Isolation and characterization of a novel human RGS mutant displaying gain-of-function activity. Cell Signal, v. 20
Doi: http://doi.org/10.1016/j.cellsig.2007.10.016

2007

Farquhar, MJ., Powner, DJ., Levine, BA., Wright, MH., Ladds, G. and Hodgkin, MN., 2007. Interaction of PLD1b with actin in antigen-stimulated mast cells. Cell Signal, v. 19
Doi: http://doi.org/10.1016/j.cellsig.2006.07.016

Allen, SCH., Moore, KAH., Marsden, CJ., Fülöp, V., Moffat, KG., Lord, JM., Ladds, G. and Roberts, LM., 2007. The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae. FEBS J, v. 274
Doi: http://doi.org/10.1111/j.1742-4658.2007.06080.x

Ladds, G., Goddard, A., Hill, C., Thornton, S. and Davey, J., 2007. Differential effects of RGS proteins on G alpha(q) and G alpha(11) activity. Cell Signal, v. 19
Doi: http://doi.org/10.1016/j.cellsig.2006.05.027

2006

Das, A., Forfar, R., Ladds, G. and Davey, J., 2006. Combined use of two transcriptional reporters improves signalling assays for G protein-coupled receptors in fission yeast. Yeast, v. 23
Doi: http://doi.org/10.1002/yea.1402

Goddard, A., Ladds, G., Forfar, R. and Davey, J., 2006. Identification of Gnr1p, a negative regulator of G alpha signalling in Schizosaccharomyces pombe, and its complementation by human G beta subunits. Fungal Genet Biol, v. 43
Doi: http://doi.org/10.1016/j.fgb.2006.06.005

Ladds, G., 2006. Activators and regulators of G protein signalling - Introduction SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, v. 17
Doi: http://doi.org/10.1016/j.semcdb.2006.03.004

Hill, C., Goddard, A., Davey, J. and Ladds, G., 2006. Investigating RGS proteins in yeast. Semin Cell Dev Biol, v. 17
Doi: http://doi.org/10.1016/j.semcdb.2006.03.008

2005

Allen, SCH., Byron, A., Lord, JM., Davey, J., Roberts, LM. and Ladds, G., 2005. Utilisation of the budding yeast Saccharomyces cerevisiae for the generation and isolation of non-lethal ricin A chain variants. Yeast, v. 22
Doi: http://doi.org/10.1002/yea.1330

Goddard, A., Ladds, G. and Davey, J., 2005. Development of a semi-quantitative plate-based alpha-galactosidase gene reporter for Schizosaccharomyces pombe and its use to isolate a constitutively active Mam2. Yeast, v. 22
Doi: http://doi.org/10.1002/yea.1190

Ladds, G., Goddard, A. and Davey, J., 2005. Functional analysis of heterologous GPCR signalling pathways in yeast. Trends Biotechnol, v. 23
Doi: http://doi.org/10.1016/j.tibtech.2005.05.007

Hiskens, R., Vatish, M., Hill, C., Davey, J. and Ladds, G., 2005. Specific in vivo binding of activator of G protein signalling 1 to the Gbeta1 subunit. Biochem Biophys Res Commun, v. 337
Doi: http://doi.org/10.1016/j.bbrc.2005.09.149

Ladds, G., Davis, K., Das, A. and Davey, J., 2005. A constitutively active GPCR retains its G protein specificity and the ability to form dimers. Mol Microbiol, v. 55
Doi: http://doi.org/10.1111/j.1365-2958.2004.04394.x

2004

Davis, K., Ladds, G., Das, A., Goddard, A. and Davey, J., 2004. An efficient bipartite PCR technique to introduce specific changes in large plasmids. Mol Biotechnol, v. 28
Doi: http://doi.org/10.1385/MB:28:3:201

Ladds, G. and Davey, J., 2004. Analysis of human GPCRs in fission yeast. Curr Opin Drug Discov Devel, v. 7

2003

Blanks, AM., Vatish, M., Allen, MJ., Ladds, G., de Wit, NCJ., Slater, DM. and Thornton, S., 2003. Paracrine oxytocin and estradiol demonstrate a spatial increase in human intrauterine tissues with labor. J Clin Endocrinol Metab, v. 88
Doi: http://doi.org/10.1210/jc.2002-021212

Ladds, G., Davis, K., Hillhouse, EW. and Davey, J., 2003. Modified yeast cells to investigate the coupling of G protein-coupled receptors to specific G proteins. Mol Microbiol, v. 47
Doi: http://doi.org/10.1046/j.1365-2958.2003.03336.x

2002

Didmon, M., Davis, K., Watson, P., Ladds, G., Broad, P. and Davey, J., 2002. Identifying regulators of pheromone signalling in the fission yeast Schizosaccharomyces pombe. Curr Genet, v. 41
Doi: http://doi.org/10.1007/s00294-002-0301-3

2000

Ladds, G., Davis, K., Powner, D. and Davey, J., 2000. A temperature-sensitive Krp1 allows in vivo characterization of kexin activation. Mol Microbiol, v. 37
Doi: http://doi.org/10.1046/j.1365-2958.2000.02028.x

Ladds, G. and Davey, J., 2000. Sxa2 is a serine carboxypeptidase that degrades extracellular P-factor in the fission yeast Schizosaccharomyces pombe. Mol Microbiol, v. 36
Doi: http://doi.org/10.1046/j.1365-2958.2000.01855.x

Ladds, G. and Davey, J., 2000. Identification of proteases with shared functions to the proprotein processing protease Krp1 in the fission yeast Schizosaccharomyces pombe. Mol Microbiol, v. 38
Doi: http://doi.org/10.1046/j.1365-2958.2000.02180.x

1998

Ladds, G., Hughes, M. and Davey, J., 1998. Extracellular degradation of agonists as an adaptive mechanism. Semin Cell Dev Biol, v. 9
Doi: http://doi.org/10.1006/scdb.1997.0215

Davey, J., Davis, K., Hughes, M., Ladds, G. and Powner, D., 1998. The processing of yeast pheromones. Semin Cell Dev Biol, v. 9
Doi: http://doi.org/10.1006/scdb.1997.0197

1997

Ladds, G. and Davey, J., 1997. Sxa2, a carboxypeptidase that degrades extracellular pheromone in fission yeast. Biochem Soc Trans, v. 25
Doi: http://doi.org/10.1042/bst025228s

1996

Ladds, G., Rasmussen, EM., Young, T., Nielsen, O. and Davey, J., 1996. The sxa2-dependent inactivation of the P-factor mating pheromone in the fission yeast Schizosaccharomyces pombe. Mol Microbiol, v. 20
Doi: http://doi.org/10.1111/j.1365-2958.1996.tb02486.x

Ladds, G. and Davey, J., 1996. Sxa2, a carboxypeptidase that degrades extracellular pheromone in the fission yeast Schizosaccharomyces pombe IMMUNOLOGY, v. 89

Conference proceedings

2021

Pearce, A., Carvalho, S. and Ladds, G., 2021. The Role of Receptor Activity Modifying Protein 3 in Glucagon-like Peptide-1 Receptor Signalling BRITISH JOURNAL OF PHARMACOLOGY, v. 178

Suchankova, A., Bowman, K., Harris, M., Rahman, T. and Ladds, G., 2021. Identification and characterisation of GIPR negative allosteric modulators BRITISH JOURNAL OF PHARMACOLOGY, v. 178

Safitri, D., Qi, H., Harris, M. and Ladds, G., 2021. Potential modulation of protease-activated receptor4 (PAR4) Threonine120 variant signalling by receptor-activity-modifying proteins (RAMPs) BRITISH JOURNAL OF PHARMACOLOGY, v. 178

Carvalho, S., Stoddart, L., Kilpatrick, L., Sabbatini, A., Soave, M., Briddon, S., Hill, S. and Ladds, G., 2021. The Adenosine A2A Receptor Does Not Appear To Undergo Agonist-Induced Internalisation BRITISH JOURNAL OF PHARMACOLOGY, v. 178

Harris, M., Truong, T., Zhao, P., Wootten, D., Sexton, P. and Ladds, G., 2021. Receptor activity modifying proteins differentially modulate G protein activation and internalisation of the gastric inhibitory polypeptide receptor. BRITISH JOURNAL OF PHARMACOLOGY, v. 178

Ladds, G., Clark, A., Wigglesworth, M. and Poyner, D., 2021. CRISPR-cas9 Interrogation of CLR signalling and the involvement of RAMPs in primary cells BRITISH JOURNAL OF PHARMACOLOGY, v. 178

Huang, X., Carvalho, S. and Ladds, G., 2021. Pharmacological and computational analysis of adenosine A2AR signal transduction BRITISH JOURNAL OF PHARMACOLOGY, v. 178

2019

Routledge, SJ., Simms, J., Dickerson, IM., Goddard, AD., Ladds, G. and Poyner, DR., 2019. RCP modulates Gas signalling of a subset of Family B GPCRs BRITISH JOURNAL OF PHARMACOLOGY, v. 176

2018

Kalash, L., Winfield, I., Safitri, D., Bermudez, M., Glen, R., Ladds, G. and Bender, A., 2018. MD-assisted approach for designing multi-target ligands at A2AR and PDE10A that elevate cyclic AMP ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, v. 256

Lazarus, K., Hadi, F., Bach, K., Watson, J., Das, M., Ladds, G., Quesne, JL., Lee, JH., Calado, D. and Khaled, W., 2018. PO-086 BCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous cell carcinoma ESMO Open, v. 3
Doi: 10.1136/esmoopen-2018-eacr25.129

2017

Kalash, L., Winfield, I., Carvalho, S., Ladds, G. and Bender, A., 2017. Rational design and evaluation of multi-target ligands at A1R, A2AR and PDE10A with therapeutic potential for neurodegenerative diseases ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, v. 254

2002

Hillhouse, EW., Randeva, H., Ladds, G. and Grammatopoulos, D., 2002. Corticotropin-releasing hormone receptors. Biochem Soc Trans, v. 30
Doi: http://doi.org/10.1042/bst0300428

1997

Ladds, G. and Davey, J., 1997. Proteolysis of Sxa2, a carboxypeptidase involved in pheromone adaptation in yeast. Biochem Soc Trans, v. 25
Doi: http://doi.org/10.1042/bst025446s

1996

Ladds, G. and Davey, J., 1996. Characterisation of Sxa2, a carboxypeptidase involved in pheromone recovery in fission yeast. Biochem Soc Trans, v. 24
Doi: http://doi.org/10.1042/bst024210s

Hughes, M., Ladds, G. and Davey, J., 1996. Defining the role of Sxa1 during pheromone adaptation in Schizosaccharomyces pombe. Biochem Soc Trans, v. 24
Doi: http://doi.org/10.1042/bst024502s

Ladds, G. and Davey, J., 1996. Purification of Sxa2, a carboxypeptidase involved in pheromone recovery in fission yeast. Biochem Soc Trans, v. 24
Doi: http://doi.org/10.1042/bst024504s

1995

Ladds, G., Rasmussen, M. and Davey, J., 1995. Characterisation of Sxa2, a protease involved in pheromone communication in fission yeast. Biochem Soc Trans, v. 23
Doi: http://doi.org/10.1042/bst023565s

Book chapters

2021

Suchankova, A., Harris, M. and Ladds, G., 2021. Measuring the rapid kinetics of receptor-ligand interactions in live cells using NanoBRET.
Doi: http://doi.org/10.1016/bs.mcb.2021.06.013

Clark, AJ. and Ladds, G., 2021. Adrenomedullin/Intermedin
Doi: 10.1007/978-3-030-57401-7_5055

2020

Clark, AJ. and Ladds, G., 2020. Adrenomedullin/Intermedin
Doi: 10.1007/978-3-030-21573-6_5055-1

2004

Ladds, G. and Davey, J., 2004. Processing Proteases in S. pombe
Doi: 10.1007/978-3-662-10360-9_26

1981

Smith, SJ. and Jackson, P., 1981. 'Introduction'

Datasets

2020 (No publication date)

Harris, M. and Ladds, G., 2020 (No publication date). Research Data File relating to the effect of chemokine receptor expression on receptor activity modifying protein expression

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2023

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2021

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2020

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2019

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2018

2017 (Accepted for publication)

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1996

2021

2019

2018

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1997

1996

1995

2021

2020

2004

1981

2020 (No publication date)

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